EMD-3333

Single-particle
4.2 Å
EMD-3333
3D View Gallery
Deposition: 14/02/2016
Map released: 04/05/2016
Last modified: 13/07/2016
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-3333
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structure of a Group II Intron Complexed with its Reverse Transcriptase

EMD-3333

Single-particle
4.2 Å
EMD-3333
3D View Gallery
Deposition: 14/02/2016
Map released: 04/05/2016
Last modified: 13/07/2016
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Grid
Details: Quantifoil Cu-Rh R1.2/1.3 grid with thin carbon support
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 1.2 µm - 3.0 µm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: CCD
Detector model: GATAN K2 SUMMIT (4k x 4k)
Number of real images: 2266
Image processing [1]
Final reconstruction
Resolution: 4.2 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 450296
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER, EMAN2, RELION - -
CTF correction
Details: Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure a of Group II Intron Complexed with its Reverse Transcriptase, Without Mask
Details: ::::EMDATABANK.org::::EMD-3333::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 200 200 200
Dimensions (Å) 261.308 261.308 261.308
Voxel size (Å) 1.30654 1.30654 1.30654
Contour list
Primary Level Source
True 0.03 AUTHOR