EMD-3283

Single-particle
7.6 Å
EMD-3283
3D View Gallery
Deposition: 17/12/2015
Map released: 27/04/2016
Last modified: 18/05/2016
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-3283
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5fua summary report (pdf.gz) Map and 5fua FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of BK polyomavirus

EMD-3283

Single-particle
7.6 Å
EMD-3283
3D View Gallery
Deposition: 17/12/2015
Map released: 27/04/2016
Last modified: 18/05/2016
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.9
Details: 10mM HEPES pH 7.9, 1mM CaCl2, 1mM MgCl2, 5mM KCl
Grid
Details: Quantifoil R2/1 EM grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Method: 6.5 seconds blot before plunging
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 0.6 µm - 4.8 µm
Nominal magnification: 19000.0
Specimen holder model: GATAN LIQUID NITROGEN
Minimum tilt angle: 0
Maximum tilt angle: 0
Image Recording [1]
Detector category: CCD
Detector model: GATAN K2 SUMMIT (4k x 4k)
Number of real images: 432
Average electron dose per image: 40 e/Å2
Details: 4 e-/A2/s, a 4 frames per second frame rate, and a 10 s exposure
Image processing [1]
Final reconstruction
Resolution: 7.6 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 2237
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
Relion - -
CTF correction
Details: CTFFIND3
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of BK polyomavirus (sharpened/masked)
Details: ::::EMDATABANK.org::::EMD-3283::::
⬡ Geometry
X Y Z
Origin -192 -192 -192
Dimensions (px) 384 384 384
Dimensions (Å) 737.27997 737.27997 737.27997
Voxel size (Å) 1.92 1.92 1.92
Contour list
Primary Level Source
True 0.022 AUTHOR