EMD-2985

Subtomogram averaging
13.0 Å
EMD-2985
3D View Gallery
Deposition: 28/04/2015
Map released: 15/07/2015
Last modified: 22/07/2015
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2985
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5a1u summary report (pdf.gz) Map and 5a1u FULL report (pdf.gz)
EMICSS annotations (xml)

The structure of the COPI coat triad

EMD-2985

Subtomogram averaging
13.0 Å
EMD-2985
3D View Gallery
Deposition: 28/04/2015
Map released: 15/07/2015
Last modified: 22/07/2015
Overview 3D View Sample Experiment Validation Additional data Links
Name: COPI coat triad on the membrane
Sample [1]
Name: COPI coat triad on the membrane
Number of unique components: 2
Details: The COPI coated vesicles were formed in an in vitro reconstituted budding reaction, which was plunge-frozen without further purification.
Molecular weight
Experimental Theoretical Method
- 1 MDa -
Protein [2]
Recombinant expression
Name: Coat protein 1 (COPI)
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Mus musculus (Mouse)
Cellular location: Golgi, cytoplasm
Molecular weight
Experimental Theoretical Method
- 560 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Spodoptera frugiperda - Sf9 pFBDM
Recombinant expression
Name: ADP-ribosylation factor 1 (Arf1)
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Saccharomyces cerevisiae (Yeast)
Cellular location: Golgi
Molecular weight
Experimental Theoretical Method
- 20 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - pOW12