EMD-29280
Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
EMD-29280
Single-particle3.48 Å
Deposition: 21/12/2022Map released: 06/09/2023
Last modified: 22/11/2023
Sample Organism:
Pseudomonas aeruginosa PA14,
synthetic construct
Sample: Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Fitted models: 8flj
Deposition Authors: Santiago-Frangos A
,
Henriques WS,
Wiegand T ,
Gauvin C,
Buyukyoruk M,
Neselu K,
Eng ET ,
Lander GC ,
Wiedenheft B
Sample: Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Fitted models: 8flj
Deposition Authors: Santiago-Frangos A
,
Henriques WS,
Wiegand T ,
Gauvin C,
Buyukyoruk M,
Neselu K,
Eng ET ,
Lander GC ,
Wiedenheft B
Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays.
Santiago-Frangos A ,
Henriques WS,
Wiegand T ,
Gauvin CC ,
Buyukyoruk M,
Graham AB ,
Wilkinson RA,
Triem L,
Neselu K,
Eng ET ,
Lander GC ,
Wiedenheft B
(2023) Nat Struct Mol Biol , 30 , 1675 - 1685
,
Henriques WS,
Wiegand T ,
Gauvin CC ,
Buyukyoruk M,
Graham AB ,
Wilkinson RA,
Triem L,
Neselu K,
Eng ET ,
Lander GC ,
Wiedenheft B
(2023) Nat Struct Mol Biol , 30 , 1675 - 1685
Grant Support:
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): GM147842 (United States)
- Life Sciences Research Foundation:
- Simons Foundation: (United States)
- M.J. Murdock Charitable Trust:
- National Science Foundation (NSF, United States): 1828765 (United States)
- NIH Common Fund Transformative High Resolution Cryo-Electron Microscopy program: GM129539
- Simons Foundation: SF349247 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): GM134867 (United States)
- Amgen:
- Montana State University Agricultural Experimental Station (USDA NIFA):
- VIRIS Detection Systems: