EMD-29214

Single-particle
3.8 Å
EMD-29214 Deposition: 18/12/2022
Map released: 29/03/2023
Last modified: 19/06/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-29214

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8fiz summary report (pdf.gz) Map-8fiz FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex)

EMD-29214

Single-particle
3.8 Å
EMD-29214 Deposition: 18/12/2022
Map released: 29/03/2023
Last modified: 19/06/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli, Escherichia phage Lambda
Sample: Cryo-EM structure of E. coli 70S Ribosome containing mRNA and tRNA (in the transcription-translation complex)
Fitted models: 8fiz

Deposition Authors: Florez Ariza A, Wee L, Tong A, Canari C, Grob P, Nogales E, Bustamante C
A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allostery.
Wee LM, Tong AB, Florez Ariza AJ , Canari-Chumpitaz C, Grob P, Nogales E, Bustamante CJ
(2023) Cell , 186 , 1244 - 1262.e34
PUBMED: 36931247
DOI: doi:10.1016/j.cell.2023.02.008
ISSN: 1097-4172