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EMD-29212

Single-particle
3.9 Å
EMD-29212
3D View Gallery
Deposition: 17/12/2022
Map released: 29/03/2023
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-29212
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8fix summary report (pdf.gz) Map and 8fix FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of E. coli RNA polymerase backtracked elongation complex harboring a terminal mismatch

EMD-29212

Single-particle
3.9 Å
EMD-29212
3D View Gallery
Deposition: 17/12/2022
Map released: 29/03/2023
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli K-12, Escherichia phage Lambda
Sample: Cryo-EM structure of E. coli RNA polymerase backtracked elongation complex harboring a terminal mismatch
Fitted models: 8fix

Deposition Authors: Florez Ariza A, Wee L, Tong A, Canari C, Grob P, Nogales E, Bustamante C
A trailing ribosome speeds up RNA polymerase at the expense of transcript fidelity via force and allostery.
Wee LM, Tong AB, Florez Ariza AJ , Canari-Chumpitaz C, Grob P, Nogales E, Bustamante CJ
(2023) Cell , 186 , 1244 - 1262.e34
PUBMED: 36931247
DOI: doi:10.1016/j.cell.2023.02.008
ISSN: 1097-4172