EMD-2896

Single-particle
13.6 Å
EMD-2896
3D View Gallery
Deposition: 10/02/2015
Map released: 06/05/2015
Last modified: 27/05/2015
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2896
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5ahv summary report (pdf.gz) Map and 5ahv FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of helical ANTH and ENTH tubules on PI(4,5)P2-containing membranes

EMD-2896

Single-particle
13.6 Å
EMD-2896
3D View Gallery
Deposition: 10/02/2015
Map released: 06/05/2015
Last modified: 27/05/2015
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Helical Array
Specimen preparation [1]
Buffer
pH: 7.5
Details: 20 mM HEPES, pH 7.5; 100 mM KCl
Grid
Details: On C-flat holey carbon coated grids (Protochips)
Vitrification
Cryogen name: ETHANE
Chamber humidity: 80%
Instrument: HOMEMADE PLUNGER
Method: , applied on C-flat holey carbon coated grids (Protochips) and vitrified
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2.7 mm
Nominal defocus: 0.5 µm - 5.0 µm
Nominal magnification: 59000.0
Calibrated magnification: 59000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 1419
Average electron dose per image: 10 e/Å2
Image processing [1]
Details: Processing done with SPRING
Final reconstruction
Resolution: 13.6 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 457344
Details:
⌯ Applied Symmetry
ΔΖ: 12.67 Å
ΔΦ: 35.857°
Axial symmetry: D2
Software [1]
Name Version Details
SPRING - -
CTF correction
Details: Each Particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of the 10.04 units per turn, 127.2 Angstrom pitch, helical assembly of ANTH-ENTH on PIP2 containing membrane
Details: ::::EMDATABANK.org::::EMD-2896::::
⬡ Geometry
X Y Z
Origin -120 -120 -170
Dimensions (px) 240 240 340
Dimensions (Å) 427.19998 427.19998 605.2
Voxel size (Å) 1.78 1.78 1.7800001
Contour list
Primary Level Source
True 0.35 AUTHOR