Happy Holidays and New year from the EMDB! Please be advised that the EMDB team will be operating at reduced capacity between December 15th - January 12th. With this in mind we would ask that you be prepared for longer reply times through both our helpdesk system and the OneDep deposition system. well done on a productive 2025, wishing you all the best and we will see you in 2026!

EMD-28665

Single-particle
3.2 Å
EMD-28665
3D View Gallery
Deposition: 26/10/2022
Map released: 01/02/2023
Last modified: 19/06/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-28665
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8exy summary report (pdf.gz) Map and 8exy FULL report (pdf.gz)
EMICSS annotations (xml)

M. tuberculosis RNAP paused complex with B. subtilis NusG and GMPCPP

EMD-28665

Single-particle
3.2 Å
EMD-28665
3D View Gallery
Deposition: 26/10/2022
Map released: 01/02/2023
Last modified: 19/06/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Mycobacterium tuberculosis H37Rv, synthetic construct, Bacillus subtilis subsp. subtilis str. 168
Sample: Pause escaped transcription complex with NusG and GMPCPP
Fitted models: 8exy

Deposition Authors: Vishwakarma RK , Murakami KS
Allosteric mechanism of transcription inhibition by NusG-dependent pausing of RNA polymerase.
Vishwakarma RK , Qayyum MZ, Babitzke P , Murakami KS
(2023) PNAS , 120 , e2218516120 - e2218516120
PUBMED: 36745813
DOI: doi:10.1073/pnas.2218516120
ISSN: 1091-6490
ASTM: PNASA6