EMD-2828

Single-particle
22.0 Å
EMD-2828
3D View Gallery
Deposition: 26/11/2014
Map released: 26/08/2015
Last modified: 26/08/2015
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2828
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structure of Mot1 in complex with TBP, NC2 and DNA

EMD-2828

Single-particle
22.0 Å
EMD-2828
3D View Gallery
Deposition: 26/11/2014
Map released: 26/08/2015
Last modified: 26/08/2015
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.05 mg/mL
Buffer
pH: 6.5
Details: 20 mM MES pH, 60 mM KCl, 5 mM MgCl2 and 2 mM DTT
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein floated on 1% w/v uranyl acetate for 20 seconds.
Grid
Details: 200 mesh carbon support grid for negative stain
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 160 kV
Nominal defocus: 0.5 µm - 1.5 µm
Calibrated magnification: 62000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Number of real images: 144
Average electron dose per image: 25 e/Å2
Image processing [1]
Details: The particles were manually selected using e2boxer
Final reconstruction
Resolution: 22.0 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 8192
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
XMIPP - -
CTF correction
Details: Micrograph level
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Negative Stain reconstruction of the Mot1 TBP NC2 complex
Details: ::::EMDATABANK.org::::EMD-2828::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 150 150 150
Dimensions (Å) 241.5 241.5 241.5
Voxel size (Å) 1.61 1.61 1.61
Contour list
Primary Level Source
True 0.03 AUTHOR