EMD-2795

Single-particle
17.0 Å
EMD-2795
3D View Gallery
Deposition: 14/10/2014
Map released: 18/02/2015
Last modified: 12/08/2015
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2795
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4v3m summary report (pdf.gz) Map and 4v3m FULL report (pdf.gz)
EMICSS annotations (xml)

Membrane bound pleurotolysin prepore (TMH2 helix lock) trapped with engineered disulphide cross-link

EMD-2795

Single-particle
17.0 Å
EMD-2795
3D View Gallery
Deposition: 14/10/2014
Map released: 18/02/2015
Last modified: 12/08/2015
Overview 3D View Sample Experiment Validation Additional data Links
Name: pleurotolysin prepore on liposomes (TMH2 helix lock) trapped with engineered disulphide
Sample [1]
Name: pleurotolysin prepore on liposomes (TMH2 helix lock) trapped with engineered disulphide
Number of unique components: 2
Details: 13 fold symmetrical ring oligomers of 26 PlyA and 13 PlyB (C487A, Y166C,G266C variant) molecules on liposomes
Protein [2]
Recombinant expression
Name: pleurotolysin A (PlyA)
Number of copies: 26
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [1]
Biological process:
symbiont-mediated hemolysis of host erythrocyte

Natural source
Organism: Pleurotus ostreatus (Oyster mushroom, White-rot fungus)
Molecular weight
Experimental Theoretical Method
- 17 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) Codon Plus pLysS (Novagen) - pET3a
Recombinant expression
Name: pleurotolysin B (PlyB)
Number of copies: 13
Details: C487A, Y166C, G266C variant
UniProtKB PDBe-KB AlphaFold DB
Domains
Gene Ontology [1]
Biological process:
symbiont-mediated hemolysis of host erythrocyte

Natural source
Organism: Pleurotus ostreatus (Oyster mushroom, White-rot fungus)
Molecular weight
Experimental Theoretical Method
- 52 MDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) Codon Plus pLysS (Novagen) - pUC57, pET3a