EMD-27142

Single-particle
2.21 Å
EMD-27142
3D View Gallery
Deposition: 30/05/2022
Map released: 20/12/2023
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-27142

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8d2l summary report (pdf.gz) Map-8d2l FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 1)

EMD-27142

Single-particle
2.21 Å
EMD-27142
3D View Gallery
Deposition: 30/05/2022
Map released: 20/12/2023
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Acidothermus cellulolyticus 11B
Sample: CryoEM Structure of AceCas9 (Cleavage intermediate 1)
Fitted models: 8d2l

Deposition Authors: Rai J, Das A , Li H
Coupled catalytic states and the role of metal coordination in Cas9.
Das A , Rai J, Roth MO , Shu Y, Medina ML , Barakat MR, Li H
(2023) Nat Catal , 6 , 969 - 977
PUBMED: 38348449
DOI: doi:10.1038/s41929-023-01031-1
ISSN: 2520-1158