EMD-26992

Single-particle
4.74 Å
EMD-26992
3D View Gallery
Deposition: 15/05/2022
Map released: 20/07/2022
Last modified: 30/11/2022
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-26992
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8ctj summary report (pdf.gz) Map and 8ctj FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of TMEM87A

EMD-26992

Single-particle
4.74 Å
EMD-26992
3D View Gallery
Deposition: 15/05/2022
Map released: 20/07/2022
Last modified: 30/11/2022
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Buffer components [2]:
Name Formula Concentration ChEBI
Tris C4H11NO3 20.0 mM
NaCl NaCl 150.0 mM
Grid
Mesh: 300
Model: Quantifoil R1.2/1.3
Material: GOLD
Support Film [1]
Material Topology Thickness
GOLD HOLEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TALOS ARCTICA
Illumination mode: FLOOD BEAM
Imaging mode: OTHER
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 0.6 µm - 1.8 µm
Image Recording [1]
Detector model: GATAN K3 (6k x 4k)
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.74 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 138217
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 180 180 180
Dimensions (Å) 200.7 200.7 200.7
Voxel size (Å) 1.115 1.115 1.115
Contour list
Primary Level Source
True 0.045 AUTHOR