EMD-2608

Single-particle
5.5 Å
EMD-2608
3D View Gallery
Deposition: 12/03/2014
Map released: 16/04/2014
Last modified: 14/05/2014
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2608
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Cryo-EM study of insect cell-expressed Enterovirus 71 and Coxsackievirus A16 virus-like particles provides a structural basis for vaccine development

EMD-2608

Single-particle
5.5 Å
EMD-2608
3D View Gallery
Deposition: 12/03/2014
Map released: 16/04/2014
Last modified: 14/05/2014
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1.7 mg/mL
Buffer
pH: 7.4
Details: 0.013M PBS
Grid
Details: Glow-discharged Quantifoil R2/1 300 mesh Cu grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.7 mm
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Image processing [1]
Final reconstruction
Resolution: 5.5 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 21403
⌯ Applied Symmetry
Point group: I
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of Coxsackievirus A16 virus-like particles
Details: ::::EMDATABANK.org::::EMD-2608::::
⬡ Geometry
X Y Z
Origin 50 50 50
Dimensions (px) 380 380 380
Dimensions (Å) 354.54 354.54 354.54
Voxel size (Å) 0.933 0.933 0.933
Contour list
Primary Level Source
True 1.0 AUTHOR