EMD-25875

Single-particle
3.25 Å
EMD-25875
3D View Gallery
Deposition: 06/01/2022
Map released: 16/11/2022
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-25875

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-7tfk summary report (pdf.gz) Map-7tfk FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

Cryo-EM 3D map of S. cerevisiae clamp loader RFC bound to two DNA molecules, one at the 5'-recessed end and the other at the 3'-recessed end

EMD-25875

Single-particle
3.25 Å
EMD-25875
3D View Gallery
Deposition: 06/01/2022
Map released: 16/11/2022
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Saccharomyces cerevisiae, synthetic construct
Sample: RFC-PCNA-DNA1-DNA2
Fitted models: 7tfk

Deposition Authors: Zheng F , Georgescu R
Cryo-EM structures reveal that RFC recognizes both the 3'- and 5'-DNA ends to load PCNA onto gaps for DNA repair.
Zheng F , Georgescu R , Yao NY, Li H , O'Donnell ME
(2022) eLife , 11
PUBMED: 35829698
DOI: doi:10.7554/eLife.77469
ISSN: 2050-084X