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EMD-24838

Single-particle
2.76 Å
EMD-24838
3D View Gallery
Deposition: 09/09/2021
Map released: 02/03/2022
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-24838
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7s4x summary report (pdf.gz) Map and 7s4x FULL report (pdf.gz)
EMICSS annotations (xml)

Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation

EMD-24838

Single-particle
2.76 Å
EMD-24838
3D View Gallery
Deposition: 09/09/2021
Map released: 02/03/2022
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: Cas9 bound to 18-20MM DNA, 1 min time-point, kinked active conformation
Fitted models: 7s4x

Deposition Authors: Bravo JPK, Taylor DW , Liu MS , Johnson KA
Structural basis for mismatch surveillance by CRISPR-Cas9.
Bravo JPK, Liu MS , Hibshman GN , Dangerfield TL, Jung K, McCool RS , Johnson KA , Taylor DW
(2022) Nature , 603 , 343 - 347
PUBMED: 35236982
DOI: doi:10.1038/s41586-022-04470-1
ISSN: 1476-4687
ASTM: NATUAS