EMD-24835

Single-particle
3.28 Å
EMD-24835
3D View Gallery
Deposition: 09/09/2021
Map released: 02/03/2022
Last modified: 14/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-24835

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-7s4v summary report (pdf.gz) Map-7s4v FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation

EMD-24835

Single-particle
3.28 Å
EMD-24835
3D View Gallery
Deposition: 09/09/2021
Map released: 02/03/2022
Last modified: 14/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation
Fitted models: 7s4v

Deposition Authors: Bravo JPK, Taylor DW , Liu MS , Johnson KA
Structural basis for mismatch surveillance by CRISPR-Cas9.
Bravo JPK, Liu MS , Hibshman GN , Dangerfield TL, Jung K, McCool RS , Johnson KA , Taylor DW
(2022) Nature , 603 , 343 - 347
PUBMED: 35236982
DOI: doi:10.1038/s41586-022-04470-1
ISSN: 1476-4687
ASTM: NATUAS