Electron Microscopy Data Bank

EMD-2320

Single-particle
10.4 Å

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Deposition: 26/02/2013
Map released: 12/03/2014
Last modified: 12/03/2014

Overview 3D View Sample Experiment Validation Additional data Links

EMD-2320

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CryoEM Structure of keyhole limpet hemocyanin isoform 2 (KLH2)

EMD-2320

Single-particle
10.4 Å
EMD-2320

3D View Gallery

Deposition: 26/02/2013
Map released: 12/03/2014
Last modified: 12/03/2014

Overview 3D View Sample Experiment Validation Additional data Links

Method: Single-particle
Aggregation State: Particle

Summary:

Specimen preparation
Microscopy
Image processing
Map

Specimen preparation [1]

Buffer

pH: 7.4
Details: 0,05 M Tris, 0,15 M NaCl, 5 mM MgCl2, 5 mM CaCl2

Vitrification

Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 100 K
Instrument: GATAN CRYOPLUNGE 3

Microscopy [1]

Microscope: FEI TECNAI F30
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 0.0013 µm - 0.0034 µm
Nominal magnification: 49000.0
Specimen holder model: GATAN LIQUID NITROGEN

Image Recording [1]

Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: PRIMESCAN
Number of real images: 210

Image processing [1]

Final reconstruction

Resolution: 10.4 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 11379
Algorithm: OTHER

Applied Symmetry

Point group: D5

Software [1]

Name	Version	Details
IMAGIC-5	-	-

CTF correction

Details: Each Particle

Map

Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of keyhole limpet hemocyanin isoform 2
Details: ::::EMDATABANK.org::::EMD-2320::::

Geometry

	X	Y	Z
Origin	-170	-170	-170
Dimensions (px)	336	336	336
Dimensions (Å)	504.0	504.0	504.0
Voxel size (Å)	1.5	1.5	1.5

Contour list

Primary	Level	Source
True	0.24	AUTHOR