EMD-2319

Single-particle
17.0 Å
EMD-2319
3D View Gallery
Deposition: 23/02/2013
Map released: 12/03/2014
Last modified: 07/05/2014
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2319
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Cryo-EM of full-length p97-FAF1 protein complex

EMD-2319

Single-particle
17.0 Å
EMD-2319
3D View Gallery
Deposition: 23/02/2013
Map released: 12/03/2014
Last modified: 07/05/2014
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.3 mg/mL
Buffer
pH: 7.4
Details: 20mM HEPES, 150mM KCl
Grid
Details: Holey carbon, quantifoil, glow discharged
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 120 K
Instrument: FEI VITROBOT MARK IV
Method: 3sec blot, 0sec drain
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.1 mm
Nominal defocus: 2.0 µm - 4.0 µm
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Nitrogen cooled
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Temperature
Minimum: 94 K
Average: 94 K
Maximum: 104 K
Image Recording [1]
Detector category: CCD
Detector model: GENERIC TVIPS
Sampling interval: 1.76 µm
Number of real images: 281
Average electron dose per image: 10 e/Å2
Bits per pixel: 16.0
Image processing [1]
Final reconstruction
Resolution: 17.0 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 4500
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C3
Software [1]
Name Version Details
Imagic - -
⦩ Final angle assignment
Details: Imagic
Final 2D classification
Number of classes: 457
CTF correction
Details: Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of full-length p97-FAF1 protein complex
Details: ::::EMDATABANK.org::::EMD-2319::::
⬡ Geometry
X Y Z
Origin -64 -64 -64
Dimensions (px) 128 128 128
Dimensions (Å) 450.56 450.56 450.56
Voxel size (Å) 3.52 3.52 3.52
Contour list
Primary Level Source
True 0.0296 AUTHOR