EMD-2144

Single-particle
17.0 Å
EMD-2144
3D View Gallery
Deposition: 26/06/2012
Map released: 22/08/2012
Last modified: 26/09/2012
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2144
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Highly Conserved Protective Epitopes on Influenza B Viruses

EMD-2144

Single-particle
17.0 Å
EMD-2144
3D View Gallery
Deposition: 26/06/2012
Map released: 22/08/2012
Last modified: 26/09/2012
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.1 mg/mL
Buffer
pH: 8.0
Details: 20 mM HEPES 8.0, 50mM NaCl
Staining
Type: NEGATIVE
Details: Grids with adsorbed protein floated on Nano-W for 30 seconds.
Grid
Details: 400 mesh gold grid with thin carbon support, glow discharged in amylamine atmosphere
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 0.6 µm - 1.2 µm
Nominal magnification: 100000.0
Calibrated magnification: 100000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 100,000 times magnification, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 55
Details: Weak beam illumination
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 10.9 µm
Number of real images: 160
Average electron dose per image: 16 e/Å2
Old range: 1.4
Bits per pixel: 16.0
Image processing [1]
Details: Particles were selected using DoG Picker and processed using a combination of EMAN2 and Sparx.
Final reconstruction
Resolution: 17.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 7116
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C3
Software [1]
Name Version Details
EMAN2, Sparx - -
CTF correction
Details: Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Influenza hemagglutinin (B/Florida/4/2006), neutralizing antibody (Fab CR8071), negative stained, single particle analysis
Details: ::::EMDATABANK.org::::EMD-2144::::
⬡ Geometry
X Y Z
Origin -80 -80 -80
Dimensions (px) 160 160 160
Dimensions (Å) 348.80002 348.80002 348.80002
Voxel size (Å) 2.18 2.18 2.18
Contour list
Primary Level Source
True 4.8 AUTHOR