EMD-2108
HRV2 empty 135S particle
EMD-2108
Single-particle9.9 Å
Deposition: 27/05/2012Map released: 14/08/2013
Last modified: 26/03/2014
Buffer
pH: 7.4
Details: 50 mM sodium borate
Details: 50 mM sodium borate
Grid
Details: R 1.2/1.3 Quantifoil grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 80%
Chamber temperature: 99 K
Instrument: LEICA EM GP
Method: Using a Leica EM Grid Plunger, sample was applied per grid in a humidified chamber, left for 30 s, blotted for 0.8 s and plunge-frozen
Chamber humidity: 80%
Chamber temperature: 99 K
Instrument: LEICA EM GP
Method: Using a Leica EM Grid Plunger, sample was applied per grid in a humidified chamber, left for 30 s, blotted for 0.8 s and plunge-frozen
Microscope: FEI TECNAI F30
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.00 mm
Nominal defocus: 0.8 µm - 3.5 µm
Nominal magnification: 71949.0
Calibrated magnification: 79372.0
Specimen holder model: PHILIPS ROTATION HOLDER
Specimen holder details: Eucentric
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal CS: 2.00 mm
Nominal defocus: 0.8 µm - 3.5 µm
Nominal magnification: 71949.0
Calibrated magnification: 79372.0
Specimen holder model: PHILIPS ROTATION HOLDER
Specimen holder details: Eucentric
Image Recording
[1]
Detector category:
CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Number of real images: 186
Average electron dose per image: 20 e/Å2
Bits per pixel: 32.0
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Sampling interval: 15 µm
Number of real images: 186
Average electron dose per image: 20 e/Å2
Bits per pixel: 32.0
Final
reconstruction
Resolution: 9.9
Å
(
BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2976
Algorithm: OTHER
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 2976
Algorithm: OTHER
⌯ Applied Symmetry
Point group:
I
Software
[1]
| Name | Version | Details |
|---|---|---|
| Xmipp | - | - |
CTF correction
Details: Phase flipping & amplitude decay correction
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of HRV2 135S empty particle
Details: ::::EMDATABANK.org::::EMD-2108::::
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of HRV2 135S empty particle
Details: ::::EMDATABANK.org::::EMD-2108::::
⬡ Geometry
| X | Y | Z | |
|---|---|---|---|
| Origin | 0 | 0 | 0 |
| Dimensions (px) | 244 | 244 | 244 |
| Dimensions (Å) | 461.16 | 461.16 | 461.16 |
| Voxel size (Å) | 1.89 | 1.89 | 1.89 |
Contour list
| Primary | Level | Source |
|---|---|---|
| True | 1.37 | AUTHOR |