EMD-20952

Single-particle
3.6 Å
EMD-20952
3D View Gallery
Deposition: 14/11/2019
Map released: 15/01/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-20952
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6uz7 summary report (pdf.gz) Map and 6uz7 FULL report (pdf.gz)
EMICSS annotations (xml)

K.lactis 80S ribosome with p/PE tRNA and eIF5B

EMD-20952

Single-particle
3.6 Å
EMD-20952
3D View Gallery
Deposition: 14/11/2019
Map released: 15/01/2020
Last modified: 06/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 6.0
Grid
Model: Quantifoil R2/2
Material: GOLD
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON CONTINUOUS 3.0
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average electron dose per image: 50.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 29712
⌯ Applied Symmetry
Point group: C1
Startup model [1]
Type: EMDB MAP
⦨ Initial angle assignment
Type: ANGULAR RECONSTITUTION
⦩ Final angle assignment
Type: ANGULAR RECONSTITUTION
Particle selection [1]
Selected Ref. model Method Software Details
64815 - - - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 400 400 400
Dimensions (Å) 428.00003 428.00003 428.00003
Voxel size (Å) 1.07 1.07 1.07
Contour list
Primary Level Source
True 0.11 AUTHOR