EMD-20612

Single-particle
4.0 Å
EMD-20612
3D View Gallery
Deposition: 16/08/2019
Map released: 26/02/2020
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-20612
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6u1n summary report (pdf.gz) Map and 6u1n FULL report (pdf.gz)
EMICSS annotations (xml)

GPCR-Beta arrestin structure in lipid bilayer

EMD-20612

Single-particle
4.0 Å
EMD-20612
3D View Gallery
Deposition: 16/08/2019
Map released: 26/02/2020
Last modified: 16/10/2024
Overview 3D View Sample Experiment Validation Additional data Links
Name: Phosphorylated human muscarinic acetylcholine receptor M2 in complex with rat beta-arrestin1, stabilized by an antibody fragment (Fab30).
Complex [4]
Name: Phosphorylated human muscarinic acetylcholine receptor M2 in complex with rat beta-arrestin1, stabilized by an antibody fragment (Fab30).
Name: Phosphorylated human muscarinic acetylcholine receptor M2
Details: Phosphorylated M2R was generated by ligating a synthetic phosphopeptide derived from the vasopressin-2-receptor (V2Rpp) using the enzyme sortase.
Natural source [1]
Organism: Homo sapiens
Name: Cysteine-free rat beta-arrestin 1 truncated at amino acid 393
Natural source [1]
Organism: Rattus norvegicus
Name: Antibody Fragment (Fab30)
Natural source [1]
Organism: Homo sapiens
Protein [4]
LEVO
Name: Muscarinic acetylcholine receptor M2, Vasopressin V2 receptor chimera
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 56 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Homo sapiens - - -
LEVO
Name: Beta-arrestin-1
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Rattus norvegicus
Molecular weight
Experimental Theoretical Method
- 45 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: Fab30 heavy chain
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 25 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
LEVO
Name: Fab30 light chain
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Homo sapiens
Molecular weight
Experimental Theoretical Method
- 23 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - -
Ligand [1]
Name: 3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide
HET code: 2CU
Number of copies: 1
ChEMBL
Molecular weight
Experimental Theoretical Method
- 437 Da -