EMD-2001

Single-particle
8.5 Å
EMD-2001
3D View Gallery
Deposition: 02/12/2011
Map released: 17/04/2012
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-2001
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 4aau summary report (pdf.gz) Map and 4aau FULL report (pdf.gz)
EMICSS annotations (xml)

ATP-triggered molecular mechanics of the chaperonin GroEL

EMD-2001

Single-particle
8.5 Å
EMD-2001
3D View Gallery
Deposition: 02/12/2011
Map released: 17/04/2012
Last modified: 24/10/2012
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 4 mg/mL
Buffer
pH: 7.4
Details: 50 mM Tris-HCl pH 7.4, 50 mM KCl, 10 mM MgCl2 and 200uM ATP
Grid
Details: Cflat grids r2/2
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 95 K
Instrument: OTHER
Method: grids were blotted for 2-3 seconds
Timed resolved state: Vitrified within 30 seconds
Details: Vitrification instrument: Vitrobot
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 0.7 µm - 3.5 µm
Calibrated magnification: 148500.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Details: The data was collected with leginon at SCRIPPS
Temperature
Minimum: 95 K
Average: 95 K
Maximum: 95 K
Image Recording [1]
Detector category: CCD
Detector model: GATAN ULTRASCAN 4000 (4k x 4k)
Average electron dose per image: 15 e/Å2
Image processing [1]
Details: The particles were automatically picked using FindEM
Final reconstruction
Resolution: 8.5 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 6500
Algorithm: OTHER
Details: SIRT was used to reconstruct the final map
⌯ Applied Symmetry
Point group: C7
Software [1]
Name Version Details
SPIDER, IMAGIC - -
⦩ Final angle assignment
Details: Theta 80-100, phi 0-51.42
CTF correction
Details: Each particle was phase flipped
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: The GroEL-ATP14 Rd1-Rd3 map is one of seven maps calculated from a heterogenous sample
Details: ::::EMDATABANK.org::::EMD-2001::::
⬡ Geometry
X Y Z
Origin -96 -96 -96
Dimensions (px) 192 192 192
Dimensions (Å) 387.84 387.84 387.84
Voxel size (Å) 2.02 2.02 2.02
Contour list
Primary Level Source
True 0.2 AUTHOR