EMD-1915

Single-particle
11.1 Å
EMD-1915
3D View Gallery
Deposition: 29/06/2011
Map released: 27/04/2012
Last modified: 11/12/2013
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-1915
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3j0d summary report (pdf.gz) Map and 3j0d FULL report (pdf.gz)
EMICSS annotations (xml)

Initial binding position of RRF on the post-termination complex

EMD-1915

Single-particle
11.1 Å
EMD-1915
3D View Gallery
Deposition: 29/06/2011
Map released: 27/04/2012
Last modified: 11/12/2013
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.08 mg/mL
Buffer
pH: 7.5
Details: 50mM Tris-HCL (pH 7.5), 10mM Mg(OAc)2, 25mM KCl
Grid
Details: 300 mesh copper grid
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 93 K
Instrument: OTHER
Details: Vitrification instrument: Vitrobot
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal defocus: 0.4 µm - 4.3 µm
Nominal magnification: 50000.0
Calibrated magnification: 50310.0
Specimen holder model: OTHER
Specimen holder details: Side entry liquid nitrogen cooled cryo holder
Alignment procedure: LEGACY (Astigmatism: Objective correct at 200kX mag, Electron beam tilt params: )
Temperature
Average: 80 K
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 14 µm
Number of real images: 195
Bits per pixel: 12.0
Image processing [1]
Final reconstruction
Resolution: 11.1 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 153927
Algorithm: OTHER
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
Final 2D classification
Number of classes: 83
CTF correction
Details: Each micrograph
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: E. coli 70S post-termination complex and Thermus thermophilus RRF.
Details: ::::EMDATABANK.org::::EMD-1915::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 130 130 130
Dimensions (Å) 361.4 361.4 361.4
Voxel size (Å) 2.78 2.78 2.78
Contour list
Primary Level Source
True 254.0 AUTHOR