Happy Holidays and New year from the EMDB! Please be advised that the EMDB team will be operating at reduced capacity between December 15th - January 12th. With this in mind we would ask that you be prepared for longer reply times through both our helpdesk system and the OneDep deposition system. well done on a productive 2025, wishing you all the best and we will see you in 2026!
EMD-18386
Archaeoglobus fulgidus AfAgo complex with AfAgo-N protein (fAfAgo) bound with 17 nt RNA guide and 17 nt DNA target
EMD-18386
Single-particle3.43 Å
Deposition: 05/09/2023Map released: 29/11/2023
Last modified: 27/03/2024
Sample Organism:
Escherichia coli,
Archaeoglobus fulgidus DSM 4304,
Archaeoglobus fulgidus
Sample: Heterodimeric AfAgo and AfAgo-N complex with 17 nt RNA guide and 17 nt DNA target
Fitted models: 8qg0
Deposition Authors: Manakova EN, Zaremba M
,
Pocevicuite R,
Golovinas E ,
Zagorskaite E ,
Silanskas A ,
Silanskas A
Sample: Heterodimeric AfAgo and AfAgo-N complex with 17 nt RNA guide and 17 nt DNA target
Fitted models: 8qg0
Deposition Authors: Manakova EN, Zaremba M
,
Pocevicuite R,
Golovinas E ,
Zagorskaite E ,
Silanskas A ,
Silanskas A
The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein.
Manakova E ,
Golovinas E ,
Poceviciute R ,
Sasnauskas G ,
Silanskas A ,
Rutkauskas D ,
Jankunec M ,
Zagorskaite E ,
Jurgelaitis E ,
Grybauskas A ,
Venclovas C ,
Zaremba M
(2024) Nucleic Acids Res , 52 , 2530 - 2545
,
Golovinas E ,
Poceviciute R ,
Sasnauskas G ,
Silanskas A ,
Rutkauskas D ,
Jankunec M ,
Zagorskaite E ,
Jurgelaitis E ,
Grybauskas A ,
Venclovas C ,
Zaremba M
(2024) Nucleic Acids Res , 52 , 2530 - 2545
Grant Support:
- Research Council of Lithuania: S-MIP-23-131 (Lithuania)
- Research Council of Lithuania: S-MIP-20-37 (Lithuania)