EMD-16303
In situ subtomogram average of GEM2 particles in human cells
EMD-16303
Subtomogram averaging31.3 Å
Deposition: 07/12/2022Map released: 09/08/2023
Last modified: 20/12/2023
Sample Organism:
Synechococcus elongatus PCC 7942 = FACHB-805,
Homo sapiens
Sample: Cryo-focused ion beam lamellae of human cells (HeLa, U2OS, Sum159) expressing the engineered genetically encoded multimeric tag GEM2
Deposition Authors: Fung HKH
,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Mueller CW ,
Cuylen-Haering S ,
Mahamid J
Sample: Cryo-focused ion beam lamellae of human cells (HeLa, U2OS, Sum159) expressing the engineered genetically encoded multimeric tag GEM2
Deposition Authors: Fung HKH
,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Mueller CW ,
Cuylen-Haering S ,
Mahamid J
Genetically encoded multimeric tags for subcellular protein localization in cryo-EM.
Fung HKH ,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Muller CW ,
Cuylen-Haering S ,
Mahamid J
(2023) Nat Methods , 20 , 1900 - 1908
,
Hayashi Y ,
Salo VT ,
Babenko A ,
Zagoriy I ,
Brunner A ,
Ellenberg J ,
Muller CW ,
Cuylen-Haering S ,
Mahamid J
(2023) Nat Methods , 20 , 1900 - 1908
Grant Support:
- German Research Foundation (DFG): 419120233 (Germany)
- EIPOD fellowship under Marie Sklodowska-Curie Actions COFUND: 664726 (Germany)
- H2020 Marie Curie Actions of the European Commission: 101028297 (European Union)
- Human Frontier Science Program (HFSP): CDA00045/2019 (France)
- Other private: Biomedicum Helsinki Foundation
- Other private: Orion Foundation