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EMD-13794
Cryo-EM of the complex between human uromodulin (UMOD)/Tamm-Horsfall protein (THP) and the FimH lectin domain from uropathogenic E. coli
EMD-13794
Single-particle7.4 Å
Deposition: 28/10/2021Map released: 16/03/2022
Last modified: 23/10/2024
Sample Organism:
Homo sapiens,
Escherichia coli (strain UTI89 / UPEC)
Sample: Complex of human uromodulin (UMOD)/Tamm-Horsfall protein (THP) and the lectin domain of the FimH adhesin of uropathogenic E. coli
Fitted models: 7q3n
Deposition Authors: Jovine L
,
Xu C
Sample: Complex of human uromodulin (UMOD)/Tamm-Horsfall protein (THP) and the lectin domain of the FimH adhesin of uropathogenic E. coli
Fitted models: 7q3n
Deposition Authors: Jovine L
,
Xu C
Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.
Stsiapanava A,
Xu C,
Nishio S ,
Han L ,
Yamakawa N ,
Carroni M,
Tunyasuvunakool K,
Jumper J ,
de Sanctis D ,
Wu B ,
Jovine L
(2022) Nat Struct Mol Biol , 29 , 190 - 193
,
Han L ,
Yamakawa N ,
Carroni M,
Tunyasuvunakool K,
Jumper J ,
de Sanctis D ,
Wu B ,
Jovine L
(2022) Nat Struct Mol Biol , 29 , 190 - 193
Secondary citations:
- Tamm I & Horsfall FL. (1950) Characterization and separation of an inhibitor of viral hemagglutination present in urine. 74, 106 - 108
- Pennica D, Kohr WJ, Kuang WJ, Glaister D, Aggarwal BB, Chen EY & Goeddel DV. (1987) Identification of human uromodulin as the Tamm-Horsfall urinary glycoprotein. Science, 236, 83 - 88
- Pak J, Pu Y, Zhang ZT, Hasty DL & Wu XR. (2001) Tamm-Horsfall protein binds to type 1 fimbriated Escherichia coli and prevents E. coli from binding to uroplakin Ia and Ib receptors. J Biol Chem, 276, 9924 - 9930
- Serafini-Cessi F, Malagolini N & Cavallone D. (2003) Tamm-Horsfall glycoprotein: biology and clinical relevance. 42, 658 - 676
- Yang H, Wu C, Zhao S & Guo J. (2004) Identification and characterization of D8C, a novel domain present in liver-specific LZP, uromodulin and glycoprotein 2, mutated in familial juvenile hyperuricaemic nephropathy. FEBS Lett, 578, 236 - 238
- Kalas V, Pinkner JS, Hannan TJ, Hibbing ME, Dodson KW, Holehouse AS, Zhang H, Tolia NH, Gross ML, Pappu RV, Janetka J & Hultgren SJ. (2017) Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions. Sci Adv, 3, e1601944
- Sauer MM, Jakob RP, Luber T, Canonica F, Navarra G, Ernst B, Unverzagt C, Maier T & Glockshuber R. (2019) Binding of the Bacterial Adhesin FimH to Its Natural, Multivalent High-Mannose Type Glycan Targets. 141, 936 - 944
- Weiss GL, Stanisich JJ, Sauer MM, Lin CW, Eras J, Zyla DS, Truck J, Devuyst O, Aebi M, Pilhofer M & Glockshuber R. (2020) Architecture and function of human uromodulin filaments in urinary tract infections. Science, 369, 1005 - 1010
- Stsiapanava A, Xu C, Brunati M, Zamora-Caballero S, Schaeffer C, Bokhove M, Han L, Hebert H, Carroni M, Yasumasu S, Rampoldi L, Wu B & Jovine L. (2020) Cryo-EM structure of native human uromodulin, a zona pellucida module polymer. EMBO J, 39, e106807
- Jumper J, Evans R, Pritzel A, Green T, Figurnov M, Ronneberger O, Tunyasuvunakool K, Bates R, Zidek A, Potapenko A, Bridgland A, Meyer C, Kohl SAA, Ballard AJ, Cowie A, Romera-Paredes B, Nikolov S, Jain R, Adler J, Back T, Petersen S, Reiman D, Clancy E, Zielinski M, Steinegger M, Pacholska M, Berghammer T, Bodenstein S, Silver D, Vinyals O, Senior AW, Kavukcuoglu K, Kohli P & Hassabis D. (2021) Highly accurate protein structure prediction with AlphaFold. Nature, 596, 583 - 589
Grant Support:
- Swedish Research Council: 2016-03999 (Singapore)
- Swedish Research Council: 2020-04936 (Singapore)
- Knut and Alice Wallenberg Foundation: 2018.0042 (Singapore)
- Ministry of Education (MoE, Singapore): MOH-000382-00 (Singapore)