EMD-1270

Single-particle
25.0 Å
EMD-1270
3D View Gallery
Deposition: 02/10/2006
Map released: 03/10/2006
Last modified: 26/05/2011
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-1270
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structural model of full-length human Ku70-Ku80 heterodimer and its recognition of DNA and DNA-PKcs.

EMD-1270

Single-particle
25.0 Å
EMD-1270
3D View Gallery
Deposition: 02/10/2006
Map released: 03/10/2006
Last modified: 26/05/2011
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.068 mg/mL
Buffer
Details: 20 mM HEPES pH 7.5, 10% glycerol, 1mM DTT, 1mM EDTA, 400mM NaCl
Staining
Type: NEGATIVE
Details: A few microliters of the purified Ku complexes were adsorbed to glow discharged carbon coated grids and negatively stained using 1% uranyl acetate.
Grid
Details: 400 mesh Copper/Palladium grid
Vitrification
Cryogen name: NONE
Microscopy [1]
Microscope: JEOL 1230
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: TUNGSTEN HAIRPIN
Acceleration voltage: 100 kV
Nominal CS: 2.9 mm
Nominal magnification: 50000.0
Specimen holder model: OTHER
Specimen holder details: Eucentric
Alignment procedure: LEGACY (Astigmatism: correction with FFT and CCD camera, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 0
Details: Microscope used: JEOL1230
Image Recording [1]
Detector category: FILM
Detector model: KODAK 4489 FILM
Scanner: OTHER
Sampling interval: 10 µm
Bits per pixel: 16.0
Details: Scanner: MINOLTA Dimage Scan Multi Pro scanner
Image processing [1]
Final reconstruction
Resolution: 25.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3419
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
EMAN - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Frontal view of the apoKu volume at a thresold of 2.5
Details: ::::EMDATABANK.org::::EMD-1270::::
⬡ Geometry
X Y Z
Origin -48 -48 -48
Dimensions (px) 96 96 96
Dimensions (Å) 203.52 203.52 203.52
Voxel size (Å) 2.12 2.12 2.12
Contour list
Primary Level Source
True 1.22 -