EMD-12604

Single-particle
3.0 Å
EMD-12604
3D View Gallery
Deposition: 15/03/2021
Map released: 16/06/2021
Last modified: 09/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-12604
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7nvh summary report (pdf.gz) Map and 7nvh FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the mycolic acid transporter MmpL3 from M. tuberculosis

EMD-12604

Single-particle
3.0 Å
EMD-12604
3D View Gallery
Deposition: 15/03/2021
Map released: 16/06/2021
Last modified: 09/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 2 mg/mL
Buffer
pH: 7.5
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K3 BIOQUANTUM (6k x 4k)
Average electron dose per image: 58.2 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 414082
Startup model [1]
Type: NONE
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
CTF correction
Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Sharpened map
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 320 320 320
Dimensions (Å) 266.24 266.24 266.24
Voxel size (Å) 0.83199996 0.83199996 0.83199996
Contour list
Primary Level Source
True 0.4 AUTHOR