EMD-1254

Single-particle
16.0 Å
EMD-1254
3D View Gallery
Deposition: 09/08/2006
Map released: 22/08/2007
Last modified: 26/05/2011
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-1254
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Hexameric ring structure of human MCM10 DNA replication factor.

EMD-1254

Single-particle
16.0 Å
EMD-1254
3D View Gallery
Deposition: 09/08/2006
Map released: 22/08/2007
Last modified: 26/05/2011
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.01 mg/mL
Buffer
pH: 6.8
Details: 25 mM Tris-HCl pH 9.0, 150 mM NaCl, 10 mM MgCl2, 50 mM KCl
Staining
Type: NEGATIVE
Details: Grids were stained with 2% w/v methylamine tungstate, (Nano-W, Nanoprobes Inc.) for 1 min.
Grid
Details: 400 mesh, freshly glow-discharged in air
Vitrification
Cryogen name: NONE
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: TUNGSTEN HAIRPIN
Acceleration voltage: 100 kV
Nominal CS: 2.2 mm
Nominal defocus: 1.5 µm - 2.5 µm
Nominal magnification: 44000.0
Specimen holder model: OTHER
Specimen holder details: Eucentric
Details: Images were taken on FEI Technai T10 microscope in low dose mode.
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: ZEISS SCAI
Sampling interval: 7 µm
Number of real images: 15
Average electron dose per image: 15 e/Å2
Detector distance: 500
Old range: 1.4
Bits per pixel: 8.0
Image processing [1]
Details: The particles were selected interactively at the computer terminal. close
Final reconstruction
Resolution: 16.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 5000
Algorithm: OTHER
Details: Final map was calculated from 197 best classes
⌯ Applied Symmetry
Point group: C6
Software [1]
Name Version Details
Imagic - -
Final 2D classification
Number of classes: 197
CTF correction
Details: Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Surface views of human Mcm10 top side bottom stereo
Details: ::::EMDATABANK.org::::EMD-1254::::
⬡ Geometry
X Y Z
Origin -60 -60 -60
Dimensions (px) 120 120 120
Dimensions (Å) 190.8 190.8 190.8
Voxel size (Å) 1.59 1.59 1.59
Contour list
Primary Level Source
True 0.0255 -