EMD-1160

Single-particle
16.0 Å
EMD-1160
3D View Gallery
Deposition: 22/09/2005
Map released: 07/08/2006
Last modified: 17/10/2012
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-1160
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Double hexameric ring assembly of the type III protein translocase ATPase HrcN.

EMD-1160

Single-particle
16.0 Å
EMD-1160
3D View Gallery
Deposition: 22/09/2005
Map released: 07/08/2006
Last modified: 17/10/2012
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 1.0 mg/mL
Buffer
pH: 7.4
Details: 50mM Tris-Cl, pH 7.4, 0.2M NaCl, 20% glycerol
Grid
Details: Quantifoil 300 mesh
Vitrification
Cryogen name: ETHANE
Instrument: HOMEMADE PLUNGER
Details: Vitrification instrument: Home-built
Microscopy [1]
Microscope: FEI/PHILIPS CM200FEG
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal magnification: 50000.0
Calibrated magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Eucentric
Maximum tilt angle: 0
Image Recording [1]
Scanner: PRIMESCAN
Sampling interval: 5.0 µm
Number of real images: 165
Old range: 1.0
Bits per pixel: 16.0
Image processing [1]
Final reconstruction
Resolution: 16.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 8742
⌯ Applied Symmetry
Point group: D6
Software [1]
Name Version Details
SPIDER - -
Final 2D classification
Number of classes: 352
CTF correction
Details: SPIDER
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: This volume contains the 3D reconstruction of dodecameric HrcN. It is D6-symmetrized. A threshold of 0.61 is recommended for surface rendering.
Details: ::::EMDATABANK.org::::EMD-1160::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 100 100 100
Dimensions (Å) 400 400 400
Voxel size (Å) 4 4 4
Contour list
Primary Level Source
True 0.802 -