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EMD-10362

Helical reconstruction
2.8 Å
EMD-10362
3D View Gallery
Deposition: 03/10/2019
Map released: 30/10/2019
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-10362
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6t17 summary report (pdf.gz) Map and 6t17 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the wild-type flagellar filament of the Firmicute Kurthia

EMD-10362

Helical reconstruction
2.8 Å
EMD-10362
3D View Gallery
Deposition: 03/10/2019
Map released: 30/10/2019
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.3
Vitrification
Cryogen name: ETHANE
Instrument: LEICA EM GP
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 86.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of classed used: 1
Number of images used: 9270
Algorithm: BACK PROJECTION
⌯ Applied Symmetry
ΔΖ: 4.83 Å
ΔΦ: 65.45°
Axial symmetry: C1
Startup model [1]
Type: OTHER
Details:Sub-tomogram average
⦩ Final angle assignment
Type: NOT APPLICABLE
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 256 256 256
Dimensions (Å) 270.848 270.848 270.848
Voxel size (Å) 1.058 1.058 1.058
Contour list
Primary Level Source
True 0.0244 AUTHOR