EMD-0583

Single-particle
3.28 Å
EMD-0583
3D View Gallery
Deposition: 17/02/2019
Map released: 10/07/2019
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-0583

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-6o0x summary report (pdf.gz) Map-6o0x FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

EMD-0583

Single-particle
3.28 Å
EMD-0583
3D View Gallery
Deposition: 17/02/2019
Map released: 10/07/2019
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: Cas9-sgRNA-DNA ternary complex
Fitted models: 6o0x

Deposition Authors: Zhu X , Clarke R
Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.
Zhu X , Clarke R, Puppala AK , Chittori S , Merk A, Merrill BJ , Simonovic M , Subramaniam S
(2019) Nat Struct Mol Biol , 26 , 679 - 685
PUBMED: 31285607
DOI: doi:10.1038/s41594-019-0258-2
ISSN: 1545-9985