Happy Holidays and New year from the EMDB! Please be advised that the EMDB team will be operating at reduced capacity between December 15th - January 12th. With this in mind we would ask that you be prepared for longer reply times through both our helpdesk system and the OneDep deposition system. well done on a productive 2025, wishing you all the best and we will see you in 2026!

EMD-0583

Single-particle
3.28 Å
EMD-0583
3D View Gallery
Deposition: 17/02/2019
Map released: 10/07/2019
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-0583
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6o0x summary report (pdf.gz) Map and 6o0x FULL report (pdf.gz)
EMICSS annotations (xml)

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

EMD-0583

Single-particle
3.28 Å
EMD-0583
3D View Gallery
Deposition: 17/02/2019
Map released: 10/07/2019
Last modified: 21/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: Cas9-sgRNA-DNA ternary complex
Fitted models: 6o0x

Deposition Authors: Zhu X , Clarke R
Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.
Zhu X , Clarke R, Puppala AK, Chittori S , Merk A, Merrill BJ , Simonovic M , Subramaniam S
(2019) Nat Struct Mol Biol , 26 , 679 - 685
PUBMED: 31285607
DOI: doi:10.1038/s41594-019-0258-2
ISSN: 1545-9985