EMDB < EMD-0391

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

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scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

EMD-0391

Single-particle
11.2 Å

EMD-0391

3D View Gallery

Deposition: 05/12/2018
Map released: 06/03/2019
Last modified: 06/03/2019

Overview 3D View Sample Experiment Validation Additional data Links

Overview 3D View Sample Experiment Validation Additional data Links

EMD-0391

Download

Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx and of gp4 (E343Q) bound to a DNA fork (Lead1)

EMD-0391

Single-particle
11.2 Å
EMD-0391

EMD-0391

3D View Gallery

Deposition: 05/12/2018
Map released: 06/03/2019
Last modified: 06/03/2019

Overview 3D View Sample Experiment Validation Additional data Links

Name: T7 DNA polymerase/Trx interacting with C-terminal tails of two helicase domains (A and E) of T7 helicase (gp4) in complex with a fork DNA substrate and dTTP (from gp4-gp5 leading-strand complex Lead1)

Summary:

Complex

T7 DNA polymerase/Trx interacting with C-terminal tails of two helicase domains (A and E) of T7 helicase (gp4) in complex with a fork DNA substrate and dTTP (from gp4-gp5 leading-strand complex Lead1)

Protein

gene product 4 helicase-primase
gene product 5 DNA polymerase
Thioredoxin 1

DNA

Primer
Template

Complex [1]

Name: T7 DNA polymerase/Trx interacting with C-terminal tails of two helicase domains (A and E) of T7 helicase (gp4) in complex with a fork DNA substrate and dTTP (from gp4-gp5 leading-strand complex Lead1)

Natural source [1]

Organism: Enterobacteria phage T7

Recombinant Expression [1]

Organism	Strain	Cell	Plasmid
Escherichia coli	-	-	-

Protein [3]

LEVO

Name: gene product 4 helicase-primase
UniProtKB PDBe-KB

Natural source

Organism: Enterobacteria phage T7

LEVO

Name: gene product 5 DNA polymerase
UniProtKB PDBe-KB

Natural source

Organism: Enterobacteria phage T7

LEVO

Name: Thioredoxin 1

Natural source

Organism: Escherichia coli

DNA [2]

DNA

Name: Primer

Natural source

Organism: Enterobacteria phage T7

DNA

Name: Template

Natural source

Organism: Enterobacteria phage T7