EMD-0180

Single-particle
2.78 Å
EMD-0180
3D View Gallery
Deposition: 10/08/2018
Map released: 12/12/2018
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-0180
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6hbc summary report (pdf.gz) Map and 6hbc FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of the repeat unit in the network formed by CcmM and Rubisco from Synechococcus elongatus

EMD-0180

Single-particle
2.78 Å
EMD-0180
3D View Gallery
Deposition: 10/08/2018
Map released: 12/12/2018
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 5 mg/mL
Details: This sample contained 10 nm gold beads and was not monodisperse
Buffer
pH: 8.0
Buffer components [4]:
Name Formula Concentration ChEBI
Potassium chloride KCl 50.0 mM
Tris (HOCH2)3CNH2 50.0 mM
Magnesium acetate Mg(CH3COO)2 10.0 mM
DTT C4H10O2S2 5.0 mM
Details: Solutions were made fresh
Grid
Mesh: 300
Model: Quantifoil R2/1
Material: COPPER
Pretreatment
Type: PLASMA CLEANING
Time: 30 s
Atmosphere: AIR
Pressure: 101.325 kPa
Support Film [1]
Material Topology Thickness
CARBON HOLEY -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 298 K
Instrument: FEI VITROBOT MARK IV
Details: Blot for 3 seconds before plunging.
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 QUANTUM (4k x 4k)
Average exposure time: 0.15 s
Average electron dose per image: 1.05 e/Å2
Image processing [1]
Final reconstruction
Resolution: 2.78 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 78916
⌯ Applied Symmetry
Point group: C1
Startup model [1]
Type: OTHER
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Map after post-processing
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 256 256 256
Dimensions (Å) 210.432 210.432 210.432
Voxel size (Å) 0.822 0.822 0.822
Contour list
Primary Level Source
True 0.0725 AUTHOR