EMD-0119

Tomography
EMD-0119
Gallery
Deposition: 10/07/2018
Map released: 16/01/2019
Last modified: 13/01/2021
Overview Sample Experiment Validation Additional data Links
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EMD-0119
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Cryo-electron tomogram of chromatin plates from metaphase chromosomes

EMD-0119

Tomography
EMD-0119
Gallery
Deposition: 10/07/2018
Map released: 16/01/2019
Last modified: 13/01/2021
Overview Sample Experiment Validation Additional data Links
Method: Tomography
Aggregation State: Particle
Specimen preparation [1]
Details: Chromatin emanated from soft-denatured metaphase chromosomes
Buffer
pH: 7.2
Details: 5 mM Pipes (pH 7.2), 5 mM NaCl, and 5 mM MgCl2
Vitrification
Cryogen name: ETHANE-PROPANE
Instrument: FEI VITROBOT MARK III
Fiducial Markers
Manufacturer Fiducial type Diameter
Aurion BSA - 10 nanometer
Sectioning
Details: NO SECTIONING
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: OTHER
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specialist optics
Phase plate: VOLTA PHASE PLATE
Energy filter
Name: GIF 2002
Image Recording [2]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 1.8 e/Å2
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: INTEGRATING
Average electron dose per image: 1.8 e/Å2
Image processing [1]
Final reconstruction
Number of images used: 59
Algorithm: BACK PROJECTION
Tomogram
Format: CCP4
Data type: IMAGE STORED AS SIGNED INTEGER (2 BYTES)
Annotation details: Cryo-electron tomogram of chromatin plates from metaphase chromosomes
⬡ Geometry
X Y Z
Origin 0 0 232
Dimensions (px) 928 928 464
Dimensions (Å) 3906.8801 3906.8801 1953.4401
Voxel size (Å) 4.21 4.21 4.21
Contour list
Primary Level Source
True - AUTHOR