› 4tvo

X-ray diffraction
1.5Å resolution

Structure of Malate Dehydrogenase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4tvo/pdb
PDB entry 4tvo coloured by chain, front view.
PDB entry 4tvo coloured by chain, side view.
PDB entry 4tvo coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structures of citrate synthase and malate dehydrogenase of Mycobacterium tuberculosis.
Ferraris DM, Spallek R, Oehlmann W, Singh M, Rizzi M
Proteins 83 389-94 (2015)
PMID: 25524525

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 34.39 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WK13 (Residues: 1-329; Coverage: 100%)
Gene names: MTV006.12, Rv1240, mdh
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand NA 22 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 52.606Å b: 79.307Å c: 155.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.193
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Mycobacterium tuberculosis Molecular Determinants of Infection, Survival Strategies, and Vulnerable Targets.
Ferraris et al. (2018)
7 other articles

3 mentions without citation

Systematic Analysis of Mycobacterial Acylation Reveals First Example of Acylation-mediated Regulation of Enzyme Activity of a Bacterial Phosphatase.
Singhal et al. (2015)
2 more