Active Site

Malate dehydrogenase, active site (IPR001252)

Short name: Malate_DH_AS


Malate dehydrogenase (EC: (MDH) [PMID: 1610875] catalyzes the interconversion of malate to oxaloacetate utilizing the NAD/NADH cofactor system. The enzyme participates in the citric acid cycle and exists in all aerobics organisms.

While prokaryotic organisms contains a single form of MDH, in eukaryotic cells there are two isozymes: one which is located in the mitochondrial matrix and the other in the cytoplasm. Fungi and plants also harbor a glyoxysomal form which functions in the glyoxylate pathway. In plants chloroplast there is an additional NADP-dependent form of MDH (EC: which is essential for both the universal C3 photosynthesis (Calvin) cycle and the more specialised C4 cycle.

The pattern for this enzyme includes two residues involved in the catalytic mechanism [PMID: 2775751]: an aspartic acid which is involved in a proton relay mechanism, and an arginine which binds the substrate.

GO terms

Biological Process

GO:0006108 malate metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0016615 malate dehydrogenase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns