› 3h45

X-ray diffraction
2.65Å resolution

Glycerol Kinase H232E with Ethylene Glycol

Released:
DOI: 10.2210/pdb3h45/pdb
PDB entry 3h45 coloured by chain, front view.
PDB entry 3h45 coloured by chain, side view.
PDB entry 3h45 coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Structural characterizations of glycerol kinase: unraveling phosphorylation-induced long-range activation.
Yeh JI, Kettering R, Saxl R, Bourand A, Darbon E, Joly N, Briozzo P, Deutscher J
Biochemistry 48 346-56 (2009)
PMID: 19102629

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128519 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: C, D, O, X
Molecule details ›
Chains: C, D, O, X
Length: 506 amino acids
Theoretical weight: 55.8 KDa
Source organism: Enterococcus casseliflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: O34153 (Residues: 1-506; Coverage: 100%)
Gene name: glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 98.606Å b: 105.194Å c: 114.305Å
α: 90° β: 114.55° γ: 90°
R-values:
R R work R free
0.257 0.208 0.251
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.
Deutscher et al. (2014)
1 more

1 mention without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)