› 5mkp

X-ray diffraction
2.5Å resolution

Non redox thiolation in transfer RNAs occuring via sulfur activation by a [4Fe-4S] cluster

Released:
DOI: 10.2210/pdb5mkp/pdb
PDB entry 5mkp coloured by chain, front view.
PDB entry 5mkp coloured by chain, side view.
PDB entry 5mkp coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster.
Arragain S, Bimai O, Legrand P, Caillat S, Ravanat JL, Touati N, Binet L, Atta M, Fontecave M, Golinelli-Pimpaneau B
Proc Natl Acad Sci U S A 114 7355-7360 (2017)
PMID: 28655838

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA-5-methyluridine(54) 2-sulfurtransferase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 35.92 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58038 (Residues: 1-310; Coverage: 100%)
Gene names: PH0300, ttuA
Sequence domains:
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand Q46 1 x Q46
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P43212
Unit cell:
a: 70.12Å b: 70.12Å c: 127.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA.
Hori et al. (2018)
6 more

2 mentions without citation

Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster.
Arragain et al. (2017)
1 more