Cytoplasmic tRNA 2-thiolation protein 1 (IPR000541)

Short name: Ncs6/Tuc1/Ctu1

Overlapping homologous superfamilies

Family relationships


Cytoplasmic tRNA 2-thiolation protein 1 (also known as Ncs6/Tuc1 in budding yeasts) is responsible for 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln) [PMID: 18664566, PMID: 18391219]. It directly bind tRNAs and probably acts by catalysing adenylation of tRNAs, an intermediate required for 2-thiolation. Its fission yeast homologue, Ctu1 forms a complex with Ctu2 (Ncs2/Tuc2 homologue) and serves as a putative enzyme for the formation of 2-thiouridine [PMID: 19151091]. This family also includes tRNA-5-methyluridine(54) 2-sulfurtransferase, which catalyzes the 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine [PMID: 28655838].

GO terms

Biological Process

GO:0034227 tRNA thio-modification
GO:0002098 tRNA wobble uridine modification

Molecular Function

GO:0000049 tRNA binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.