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Structure of the native microtubule lattice nucleated from the yeast spindle pole body
Resolution: 6.6 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv0
Q-score: 0.123
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01281-y Pubmed: 38609662 ]
Resolution: 9.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv2
Q-score: 0.112
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01281-y Pubmed: 38609662 ]
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Unkown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Y-tubulin ring complex capping the microtubule minus end (Cellular component from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
Resolution: 8.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv3
Q-score: 0.144
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01281-y Pubmed: 38609662 ]
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Y-tubulin small complex bound to two alpha/beta tubulin dimers (Cellular component from Saccharomyces cerevisiae)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
D. melanogaster 13-protofilament microtubule
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7qup
Q-score: 0.46
Wagstaff JM, Planelles-Herrero VJ, Sharov G, Alnami A, Kozielski F, Derivery E, Lowe J
Sci Adv (2023) 9 pp. eadf3021-eadf3021 [ Pubmed: 36989372 DOI: doi:10.1126/sciadv.adf3021 ]
- Tubulin beta-1 chain (47 kDa, Protein from Drosophila melanogaster)
- Microtubule (1 MDa, Complex from Drosophila melanogaster)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1 chain (52 kDa, Protein from Drosophila melanogaster)
D. melanogaster alpha/beta tubulin heterodimer in the GDP form
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7quc
Q-score: 0.509
Wagstaff JM, Planelles-Herrero VJ, Sharov G, Alnami A, Kozielski F, Derivery E, Lowe J
Sci Adv (2023) 9 pp. eadf3021-eadf3021 [ Pubmed: 36989372 DOI: doi:10.1126/sciadv.adf3021 ]
- Tubulin alpha-1 chain (Complex from Drosophila melanogaster)
- Tubulin beta-1 chain (Complex from Drosophila melanogaster)
- Tubulin beta-1 chain (50 kDa, Protein from Drosophila melanogaster)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Alpha1/beta tubulin heterodimer (Complex)
- Tubulin alpha-1 chain (52 kDa, Protein from Drosophila melanogaster)
D. melanogaster alpha/beta tubulin heterodimer in the GTP form
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7qud
Q-score: 0.46
Wagstaff JM, Planelles-Herrero VJ, Sharov G, Alnami A, Kozielski F, Derivery E, Lowe J
Sci Adv (2023) 9 pp. eadf3021-eadf3021 [ Pubmed: 36989372 DOI: doi:10.1126/sciadv.adf3021 ]
- Tubulin alpha-1 chain (Complex from Drosophila melanogaster)
- Tubulin beta-1 chain (Complex from Drosophila melanogaster)
- Tubulin beta-1 chain (50 kDa, Protein from Drosophila melanogaster)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Alpha1/beta tubulin heterodimer (Complex)
- Tubulin alpha-1 chain (52 kDa, Protein from Drosophila melanogaster)
S. pombe microtubule decorated with Cut7 motor domain in the AMPPNP state
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5mlv
Q-score: 0.355
von Loeffelholz O, Pena A, Drummond DR, Cross R, Moores CA
J Mol Biol (2019) 431 pp. 864-872 [ DOI: doi:10.1016/j.jmb.2019.01.011 Pubmed: 30659798 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Cut7 mtd decorated s. pombe microtubule stabilized with epothilone-b (Complex)
- Alpha tubulin, beta tubulin (Complex from Schizosaccharomyces pombe 972)
- Kinesin-like protein cut7 (47 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Tubulin beta chain (49 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 7,11-dihydroxy-8,8,10,12,16-pentamethyl-3-[1-methyl-2-(2-methyl-thiazol-4-yl)vinyl]-4,17-dioxabicyclo[14.1.0]heptadecane-5,9-dione (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1 chain (51 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Cut7 (Complex from Schizosaccharomyces pombe 972)
S. pombe microtubule copolymerized with GTP and Mal3-143
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 5mjs
Q-score: 0.331
von Loeffelholz O, Venables NA, Drummond DR, Katsuki M, Cross R, Moores CA
Nat Commun (2017) 8 pp. 2110-2110 [ DOI: doi:10.1038/s41467-017-02241-5 Pubmed: 29235477 ]
- Tubulin alpha-1 chain (49 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Microtubule integrity protein mal3 (16 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Tubulin beta chain (47 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Guanosine-5'-diphosphate (443 Da, Ligand from Schizosaccharomyces pombe)
- Microtubule decorated with mal3 (Complex from Schizosaccharomyces pombe)
- Guanosine-5'-triphosphate (523 Da, Ligand from Schizosaccharomyces pombe)
Yeast tubulin polymerized with GTP in vitro
Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3f
Q-score: 0.366
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dynamic microtubule lattice (Complex from Saccharomyces cerevisiae S288c)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast microtubule stabilized with epothilone
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3h
Q-score: 0.369
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Microtubule lattice stabilized with epothilone (Complex from Saccharomyces cerevisiae S288c)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Epothilone a (493 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))