› 5w3h

Electron Microscopy
4Å resolution

Yeast microtubule stabilized with epothilone

Released:
DOI: 10.2210/pdb5w3h/pdb
PDB entry 5w3h coloured by chain, front view.
PDB entry 5w3h coloured by chain, side view.
PDB entry 5w3h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural differences between yeast and mammalian microtubules revealed by cryo-EM.
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol 216 2669-2677 (2017)
PMID: 28652389
Related structures: EMD-8756

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135931 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin alpha-1 chain Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.85 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P09733 (Residues: 1-447; Coverage: 100%)
Gene names: TUB1, YML085C
Sequence domains:
Structure domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 457 amino acids
Theoretical weight: 50.97 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P02557 (Residues: 1-457; Coverage: 100%)
Gene names: TUB2, YFL037W
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand EP 1 x EP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-8756

Quick links

Citations

10 review citations

The tubulin code and its role in controlling microtubule properties and functions.
Janke et al. (2020)
9 more

3 mentions without citation

Structural differences between yeast and mammalian microtubules revealed by cryo-EM.
Howes et al. (2017)
2 more