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Cryo-EM structure of P-Rex1-IP4
Ravala SK, Adame-Garcia SR, Li S, Chen CL, Cianfrocco MA, Gutkind JS, Cash JN, Tesmer JJG
eLife (2024) [ DOI: 10.7554/eLife.92822.1 ]
Image sets:
Full-length P-Rex1 in complex with inositol 1,3,4,5-tetrakisphosphate (IP4)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8tua
Q-score: 0.306
Ravala SK, Adame-Garcia SR, Li S, Chen C, Cianfrocco MA, Gutkind JS, Cash JN, Tesmer JJG
eLife (2024) [ DOI: doi:10.7554/eLife.92822.1 ]
- Inositol-(1,3,4,5)-tetrakisphosphate (500 Da, Ligand)
- Complex of full-length phosphatidylinositol 3,4,5-trisphosphate (pip3)-dependent rac exchanger 1 (p-rex1) bound to inositol 1,3,4,5-tetrakisphosphate (ip4) (183 kDa, Complex from Homo sapiens)
- Phosphatidylinositol 3,4,5-trisphosphate-dependent rac exchanger 1 protein (183 kDa, Protein from Homo sapiens)
Phosphoinositide phosphate 3 kinase gamma
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8so9
Q-score: 0.514
Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01265-y Pubmed: 38565696 ]
Phosphoinositide phosphate 3 kinase gamma bound with ATP
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8soa
Q-score: 0.425
Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01265-y Pubmed: 38565696 ]
- Pi3k-gamma-atp (210 MDa, Complex from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Phosphatidylinositol-4,5-bisphosphate 3-kinase (127 kDa, Protein from Sus scrofa)
- Phosphoinositide 3-kinase regulatory subunit 5 (98 kDa, Protein from Sus scrofa)
Phosphoinositide phosphate 3 kinase gamma bound with ADP
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8sob
Q-score: 0.37
Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01265-y Pubmed: 38565696 ]
- Phosphoinositide 3-kinase regulatory subunit 5 (98 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphatidylinositol-4,5-bisphosphate 3-kinase (127 kDa, Protein from Sus scrofa)
- Pi3k-gamma-adpbef3 (210 MDa, Complex from Sus scrofa)
Phosphoinositide phosphate 3 kinase gamma bound with ADP and Gbetagamma
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8soc
Q-score: 0.337
Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01265-y Pubmed: 38565696 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Bos taurus)
- Pi3k-gamma-adp bound with gbetagamma (210 MDa, Complex from Sus scrofa)
- Phosphatidylinositol-4,5-bisphosphate 3-kinase (127 kDa, Protein from Sus scrofa)
- Phosphoinositide 3-kinase regulatory subunit 5 (98 kDa, Protein from Sus scrofa)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (8 kDa, Protein from Bos taurus)
Phosphoinositide phosphate 3 kinase gamma bound with ADP and two Gbetagamma subunits in State 1
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8sod
Q-score: 0.268
Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01265-y Pubmed: 38565696 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Pi3k-gamma-adp bound with two gbetagamma subunits in state 1 (210 MDa, Complex from Sus scrofa)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Bos taurus)
- Phosphatidylinositol-4,5-bisphosphate 3-kinase (127 kDa, Protein from Sus scrofa)
- Phosphoinositide 3-kinase regulatory subunit 5 (98 kDa, Protein from Sus scrofa)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (8 kDa, Protein from Bos taurus)
Phosphoinositide phosphate 3 kinase gamma bound with ADP and two Gbetagamma subunits in State 2
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8soe
Q-score: 0.254
Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01265-y Pubmed: 38565696 ]
- Pi3k-gamma-adp bound with two gbetagamma subunits in state 2 (210 MDa, Complex from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Bos taurus)
- Phosphatidylinositol-4,5-bisphosphate 3-kinase (127 kDa, Protein from Sus scrofa)
- Phosphoinositide 3-kinase regulatory subunit 5 (98 kDa, Protein from Sus scrofa)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (8 kDa, Protein from Bos taurus)
Human Trio residues 1284-1959 in complex with Rac1
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 7sj4
Q-score: 0.515
Bandekar SJ, Chen CL, Ravala SK, Cash JN, Avramova LV, Zhalnina MV, Gutkind JS, Li S, Tesmer JJG
J Biol Chem (2022) 298 pp. 102209-102209 [ DOI: doi:10.1016/j.jbc.2022.102209 Pubmed: 35779635 ]
- Water (18 Da, Ligand)
- Triple functional domain protein (75 kDa, Protein from Homo sapiens)
- Human trio residues 1284-1959 in complex with rac1 (Complex from Homo sapiens)
- Ras-related c3 botulinum toxin substrate 1 (21 kDa, Protein from Homo sapiens)
Cryo-electron microscopy structure of the P-Rex1–G-beta-gamma signaling scaffold
Cash JN, Urata S, Li S, Ravala SK, Avramova LV, Shost MD, Gutkind JS, Tesmer JJG, Cianfrocco MA
Sci Adv (2019) 5 [ DOI: 10.1126/sciadv.aax8855 Pubmed: 31663027 ]
Image sets: