› 8sod

Electron Microscopy
3.4Å resolution

Phosphoinositide phosphate 3 kinase gamma bound with ADP and two Gbetagamma subunits in State 1

Released:
DOI: 10.2210/pdb8sod/pdb
PDB entry 8sod coloured by chain, front view.
PDB entry 8sod coloured by chain, side view.
PDB entry 8sod coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for Gβγ-mediated activation of phosphoinositide 3-kinase γ.
Chen CL, Syahirah R, Ravala SK, Yen YC, Klose T, Deng Q, Tesmer JJG
Nat Struct Mol Biol (2024)
PMID: 38565696
Related structures: EMD-40654

Function and Biology Details

Reactions catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-242501 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chains: C, E
Molecule details ›
Chains: C, E
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform Chain: A
Molecule details ›
Chain: A
Length: 1108 amino acids
Theoretical weight: 127.57 KDa
Source organism: Sus scrofa
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O02697 (Residues: 2-1102; Coverage: 100%)
Gene name: PIK3CG
Sequence domains:
Phosphoinositide 3-kinase regulatory subunit 5 Chain: B
Molecule details ›
Chain: B
Length: 890 amino acids
Theoretical weight: 98.5 KDa
Source organism: Sus scrofa
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O02696 (Residues: 1-877; Coverage: 100%)
Gene name: PIK3R5
Sequence domains: Phosphoinositide 3-kinase gamma adapter protein p101 subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chains: D, F
Molecule details ›
Chains: D, F
Length: 77 amino acids
Theoretical weight: 8.67 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-40654
Expression system: Spodoptera frugiperda

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