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48-nm doublet microtubule from Tetrahymena thermophila strain MEC17
Yang SK, Kubo S, Black CS, Peri K, Dai D, Legal T, Valente-Paterno M, Gaertig J, Bui KH
eLife (2024) 12 [ Pubmed: 38598282 DOI: doi:10.7554/eLife.92219 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cilia- and flagella-associated protein 52 (73 kDa, Protein from Tetrahymena thermophila)
- Rib26 (26 kDa, Protein from Tetrahymena thermophila)
- Stpg1a (42 kDa, Protein from Tetrahymena thermophila)
- Cfap210 (61 kDa, Protein from Tetrahymena thermophila)
- Cfap182b (11 kDa, Protein from Tetrahymena thermophila)
- 48-nm repeat unit of microtubule doublet from tetrahymena thermophila strain mec17 (7 MDa, Cellular component from Tetrahymena thermophila)
- Cfap20 (22 kDa, Protein from Tetrahymena thermophila)
- Cfap112a (30 kDa, Protein from Tetrahymena thermophila)
- Nebulin (61 kDa, Protein from Tetrahymena thermophila)
- Ij34 (34 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 53 (60 kDa, Protein from Tetrahymena thermophila)
- Cfap21a (48 kDa, Protein from Tetrahymena thermophila)
- Cfap129 (31 kDa, Protein from Tetrahymena thermophila)
- Rib22 (22 kDa, Protein from Tetrahymena thermophila)
- Cfap182a (20 kDa, Protein from Tetrahymena thermophila)
- Rib38 (38 kDa, Protein from Tetrahymena thermophila)
- Cfap143 (30 kDa, Protein from Tetrahymena thermophila)
- Oj2 (19 kDa, Protein from Tetrahymena thermophila)
- Protofilament ribbon protein (34 kDa, Protein from Tetrahymena thermophila)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrgb (28 kDa, Protein from Tetrahymena thermophila)
- Rib57 (57 kDa, Protein from Tetrahymena thermophila)
- Cfam166b (17 kDa, Protein from Tetrahymena thermophila)
- Cfap112b (32 kDa, Protein from Tetrahymena thermophila)
- Rib27a (27 kDa, Protein from Tetrahymena thermophila)
- Dna polymerase delta c4-type zinc-finger protein (31 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Sb1 (17 kDa, Protein from Tetrahymena thermophila)
- Rib27b (26 kDa, Protein from Tetrahymena thermophila)
- Cfap107 (21 kDa, Protein from Tetrahymena thermophila)
- Cfam166c (25 kDa, Protein from Tetrahymena thermophila)
- Rib43a protein (17 kDa, Protein from Tetrahymena thermophila)
- Flagellar flij protein (12 kDa, Protein from Tetrahymena thermophila)
- Stpg2 (33 kDa, Protein from Tetrahymena thermophila)
- Cfap127 (55 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 45 (59 kDa, Protein from Tetrahymena thermophila)
- Nucleoside diphosphate kinase (42 kDa, Protein from Tetrahymena thermophila)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Parkin co-regulated protein pacrga (36 kDa, Protein from Tetrahymena thermophila)
- Rib35 (34 kDa, Protein from Tetrahymena thermophila)
- Oj3 (19 kDa, Protein from Tetrahymena thermophila)
- Flagellar microtugule protofilament ribbon protein (72 kDa, Protein from Tetrahymena thermophila)
- Cfam166a (25 kDa, Protein from Tetrahymena thermophila)
- Cfap115 (110 kDa, Protein from Tetrahymena thermophila)
- Cfap77a (28 kDa, Protein from Tetrahymena thermophila)
- Rib72b (60 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Cfap161a (57 kDa, Protein from Tetrahymena thermophila)
Combined linker domain of N-DRC and associated proteins Tetrahymena
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tid
Q-score: 0.072
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 5741-5741 [ Pubmed: 37714832 DOI: doi:10.1038/s41467-023-41480-7 ]
- Wd40 domain protein (152 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil protein, putative (60 kDa, Protein from Tetrahymena thermophila)
- Dynein regulatory complex protein 1/2 n-terminal domain-containing protein (98 kDa, Protein from Tetrahymena thermophila)
- Flagella associated protein (114 kDa, Protein from Tetrahymena thermophila)
- Cfap20 (22 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil domain-containing protein 153 (22 kDa, Protein from Tetrahymena thermophila)
- Lrrc48 protein (68 kDa, Protein from Tetrahymena thermophila)
- Growth-arrest-specific microtubule-binding protein (56 kDa, Protein from Tetrahymena thermophila)
- Flagellar associated protein (42 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil protein, putative (105 kDa, Protein from Tetrahymena thermophila)
- Duf4201 domain-containing protein (94 kDa, Protein from Tetrahymena thermophila)
- Dynein regulatory complex protein 9 (44 kDa, Protein from Tetrahymena thermophila)
- Dynein regulatory complex protein 10 (51 kDa, Protein from Tetrahymena thermophila)
- Ef hand protein (124 kDa, Protein from Tetrahymena thermophila)
- Growth-arrest-specific microtubule-binding protein (55 kDa, Protein from Tetrahymena thermophila)
- Aaa family atpase cdc48 subfamily protein (101 kDa, Protein from Tetrahymena thermophila)
- Kinase domain protein (39 kDa, Protein from Tetrahymena thermophila)
- Calmodulin 7-2 (23 kDa, Protein from Tetrahymena thermophila)
- Flagellar associated protein (52 kDa, Protein from Tetrahymena thermophila)
- Combined linker domain of n-drc and associated proteins tetrahymena (Cell from Tetrahymena thermophila)
- Ef-hand calcium-binding domain protein (22 kDa, Protein from Tetrahymena thermophila)
- L-shape (unknown-protein) (50 kDa, Protein from Tetrahymena thermophila)
- Wd40 domain protein (151 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil lobo-like protein, putative (101 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 91 (76 kDa, Protein from Tetrahymena thermophila)
Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tek
Q-score: 0.273
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 5741-5741 [ Pubmed: 37714832 DOI: doi:10.1038/s41467-023-41480-7 ]
- Growth-arrest-specific microtubule-binding protein (56 kDa, Protein from Tetrahymena thermophila)
- Dynein regulatory complex protein 1/2 n-terminal domain-containing protein (98 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil protein, putative (60 kDa, Protein from Tetrahymena thermophila)
- Flagellar associated protein (42 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil protein, putative (105 kDa, Protein from Tetrahymena thermophila)
- Duf4201 domain-containing protein (94 kDa, Protein from Tetrahymena thermophila)
- Baseplate of nexin-dynein regulatory complex from tetrahymena thermophila (Cellular component from Tetrahymena thermophila)
- Flagella associated protein (114 kDa, Protein from Tetrahymena thermophila)
- Cfap20 (22 kDa, Protein from Tetrahymena thermophila)
- Growth-arrest-specific microtubule-binding protein (55 kDa, Protein from Tetrahymena thermophila)
- Cilia- and flagella-associated protein 91 (76 kDa, Protein from Tetrahymena thermophila)
Linker domain of Nexin-dynein regulatory complex from Tetrahymena thermophila
Resolution: 7.4 Å
EM Method: Single-particle
Fitted PDBs: 8th8
Q-score: 0.087
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 5741-5741 [ Pubmed: 37714832 DOI: doi:10.1038/s41467-023-41480-7 ]
- Growth-arrest-specific microtubule-binding protein (56 kDa, Protein from Tetrahymena thermophila)
- Dynein regulatory complex protein 1/2 n-terminal domain-containing protein (98 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil protein, putative (60 kDa, Protein from Tetrahymena thermophila)
- Aaa family atpase cdc48 subfamily protein (101 kDa, Protein from Tetrahymena thermophila)
- Duf4201 domain-containing protein (94 kDa, Protein from Tetrahymena thermophila)
- Kinase domain protein (39 kDa, Protein from Tetrahymena thermophila)
- Dynein regulatory complex protein 9 (44 kDa, Protein from Tetrahymena thermophila)
- Calmodulin 7-2 (23 kDa, Protein from Tetrahymena thermophila)
- Dynein regulatory complex protein 10 (51 kDa, Protein from Tetrahymena thermophila)
- Linker domain of nexin-dynein regulatory complex from tetrahymena thermophila (Cell from Tetrahymena thermophila)
- Flagellar associated protein (52 kDa, Protein from Tetrahymena thermophila)
- Ef-hand calcium-binding domain protein (22 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil domain-containing protein 153 (22 kDa, Protein from Tetrahymena thermophila)
- Lrrc48 protein (68 kDa, Protein from Tetrahymena thermophila)
- Coiled-coil lobo-like protein, putative (101 kDa, Protein from Tetrahymena thermophila)
- Growth-arrest-specific microtubule-binding protein (55 kDa, Protein from Tetrahymena thermophila)
Focused refinement of the N-DRC from the Tetrahymena WT subtomo
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 5741-5741 [ Pubmed: 37714832 DOI: doi:10.1038/s41467-023-41480-7 ]
Linker domain of N-DRC complex from Tetrahymena thermophila
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 5741-5741 [ Pubmed: 37714832 DOI: doi:10.1038/s41467-023-41480-7 ]
96nm repeat of Doublet microtubule from Tetrahymena thermophila
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 5741-5741 [ Pubmed: 37714832 DOI: doi:10.1038/s41467-023-41480-7 ]
DRC9/10 baseplate from Tetrahymena thermophila
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun (2023) 14 pp. 5741-5741 [ Pubmed: 37714832 DOI: doi:10.1038/s41467-023-41480-7 ]
Legal T, Parra M, Tong M, Black CS, Joachimiak E, Valente-Paterno M, Lechtreck K, Gaertig J, Bui KH
J Cell Biol (2023) 222 [ Pubmed: 37756660 DOI: doi:10.1083/jcb.202301129 ]
Ciliary cap complex from Tetrahymena thermophila
Legal T, Parra M, Tong M, Black CS, Joachimiak E, Valente-Paterno M, Lechtreck K, Gaertig J, Bui KH
J Cell Biol (2023) 222 [ Pubmed: 37756660 DOI: doi:10.1083/jcb.202301129 ]