› 8tid

Electron Microscopy
3.6Å resolution

Combined linker domain of N-DRC and associated proteins Tetrahymena

Released:
DOI: 10.2210/pdb8tid/pdb
PDB entry 8tid coloured by chain, front view.
PDB entry 8tid coloured by chain, side view.
PDB entry 8tid coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism.
Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH
Nat Commun 14 5741 (2023)
PMID: 37714832
Related structures: EMD-41284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
PDBe Complex ID:
PDB-CPX-229671 (preferred)
Entry contents:
24 distinct polypeptide molecules
Macromolecules (24 distinct):
Dynein regulatory complex protein 1/2 N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 826 amino acids
Theoretical weight: 98.84 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q229S1 (Residues: 1-826; Coverage: 100%)
Gene name: TTHERM_01345750
Sequence domains: Sperm tail
Dynein regulatory complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 506 amino acids
Theoretical weight: 60.6 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q24DJ0 (Residues: 1-506; Coverage: 100%)
Gene name: TTHERM_00971830
Sequence domains: Sperm tail
Dynein regulatory complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 575 amino acids
Theoretical weight: 68.01 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MG46 (Residues: 1-575; Coverage: 100%)
Gene name: TTHERM_00316370
Sequence domains: Leucine Rich repeats (2 copies)
Growth arrest-specific protein 8 domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 472 amino acids
Theoretical weight: 56.19 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7LT80 (Residues: 1-472; Coverage: 100%)
Gene name: TTHERM_00649240
Sequence domains: Growth-arrest specific micro-tubule binding
Growth arrest-specific protein 8 domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 468 amino acids
Theoretical weight: 55.39 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23YW7 (Residues: 1-468; Coverage: 100%)
Gene name: TTHERM_00857910
Sequence domains: Growth-arrest specific micro-tubule binding
Flagellar associated protein Chain: F
Molecule details ›
Chain: F
Length: 461 amino acids
Theoretical weight: 52.94 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q24C31 (Residues: 1-461; Coverage: 100%)
Gene name: TTHERM_00697450
Sequence domains: Leucine Rich repeat
Kinase domain protein Chain: G
Molecule details ›
Chain: G
Length: 345 amino acids
Theoretical weight: 39.71 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q24CL2 (Residues: 1-345; Coverage: 100%)
Gene name: TTHERM_01220370
Sequence domains: Leucine Rich repeat
Coiled-coil lobo-like protein, putative Chain: H
Molecule details ›
Chain: H
Length: 852 amino acids
Theoretical weight: 101.23 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MLZ4 (Residues: 1-852; Coverage: 100%)
Gene name: TTHERM_00473320
EF-hand domain-containing protein Chains: I, i
Molecule details ›
Chains: I, i
Length: 185 amino acids
Theoretical weight: 22.06 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: W7WX86 (Residues: 1-185; Coverage: 100%)
Gene name: TTHERM_001232262
Dynein regulatory complex protein 9 Chain: J
Molecule details ›
Chain: J
Length: 372 amino acids
Theoretical weight: 44.99 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23S05 (Residues: 1-372; Coverage: 100%)
Gene name: TTHERM_00625950
Dynein regulatory complex protein 10 Chain: K
Molecule details ›
Chain: K
Length: 434 amino acids
Theoretical weight: 51.32 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A4VD15 (Residues: 1-434; Coverage: 100%)
Gene name: TTHERM_00535929
ATPase AAA-type core domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 862 amino acids
Theoretical weight: 101.14 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7LWE3 (Residues: 1-862; Coverage: 100%)
Gene name: TTHERM_00151820
Sequence domains: ATPase family associated with various cellular activities (AAA)
Cilia- and flagella-associated protein 91 Chain: M
Molecule details ›
Chain: M
Length: 644 amino acids
Theoretical weight: 76.15 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7LWP7 (Residues: 1-644; Coverage: 100%)
Gene name: TTHERM_00578560
Sequence domains: Cilia- and flagella-associated protein 91
Flagella associated protein Chains: N, n
Molecule details ›
Chains: N, n
Length: 963 amino acids
Theoretical weight: 114.16 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23BW0 (Residues: 1-963; Coverage: 100%)
Gene name: TTHERM_00227220
Coiled-coil protein, putative Chains: O, o
Molecule details ›
Chains: O, o
Length: 893 amino acids
Theoretical weight: 105.2 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q233L0 (Residues: 1-893; Coverage: 100%)
Gene name: TTHERM_00391400
Sequence domains: BRE1 E3 ubiquitin ligase
Cilia- and flagella-associated protein 184 Chain: P
Molecule details ›
Chain: P
Length: 794 amino acids
Theoretical weight: 94.54 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M6D6 (Residues: 1-794; Coverage: 100%)
Gene names: CCDC96, CFAP184, TTHERM_00529650
Sequence domains: Domain of unknown function (DUF4201)
EF-hand domain-containing protein Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 202 amino acids
Theoretical weight: 23.86 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MDA9 (Residues: 1-202; Coverage: 100%)
Gene name: TTHERM_00549500
Sequence domains: EF-hand domain
Dynein regulatory complex protein 12 Chains: S, s
Molecule details ›
Chains: S, s
Length: 187 amino acids
Theoretical weight: 22.17 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22RH5 (Residues: 1-187; Coverage: 100%)
Gene names: DRC12, TTHERM_00016120
Cilia- and flagella-associated protein 263 Chain: T
Molecule details ›
Chain: T
Length: 361 amino acids
Theoretical weight: 42.93 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22KK0 (Residues: 1-361; Coverage: 100%)
Gene names: CCDC113, CFAP263, TTHERM_00312810
Sequence domains: Domain of unknown function (DUF4201)
L-shape (Unknown-protein) Chains: U, V
Molecule details ›
Chains: U, V
Length: 597 amino acids
Theoretical weight: 50.83 KDa
Source organism: Tetrahymena thermophila
Cilia- and flagella-associated protein 57 C Chain: W
Molecule details ›
Chain: W
Length: 1308 amino acids
Theoretical weight: 151.72 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: W7WWA2 (Residues: 1-1308; Coverage: 100%)
Gene names: CFAP57C, TTHERM_000681920, WDR65C
Sequence domains: WD domain, G-beta repeat
Cilia- and flagella-associated protein 337 A Chain: Z
Molecule details ›
Chain: Z
Length: 1079 amino acids
Theoretical weight: 124.97 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MM07 (Residues: 1-1079; Coverage: 100%)
Gene names: CFAP337A, TTHERM_00475060
Sequence domains: WD domain, G-beta repeat
Cilia- and flagella-associated protein 20 Chain: g
Molecule details ›
Chain: g
Length: 195 amino acids
Theoretical weight: 22.98 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22NU3 (Residues: 1-195; Coverage: 100%)
Gene name: TTHERM_00418580
Sequence domains: CFA20 domain
Cilia- and flagella-associated protein 57 A Chain: w
Molecule details ›
Chain: w
Length: 1293 amino acids
Theoretical weight: 152.7 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q234G8 (Residues: 1-1293; Coverage: 100%)
Gene names: CFAP57A, TTHERM_00105300, WDR65C
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-41284

Quick links

Citations

1 review citation

Structure and Function of Dynein's Non-Catalytic Subunits.
Rao et al. (2024)