› 7lyb

Electron Microscopy
3.28Å resolution

Cryo-EM structure of the human nucleosome core particle in complex with BRCA1-BARD1-UbcH5c

Released:
DOI: 10.2210/pdb7lyb/pdb
PDB entry 7lyb coloured by chain, front view.
PDB entry 7lyb coloured by chain, side view.
PDB entry 7lyb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanisms of BRCA1-BARD1 nucleosome recognition and ubiquitylation.
Hu Q, Botuyan MV, Zhao D, Cui G, Mer E, Mer G
Nature 596 438-443 (2021)
PMID: 34321665
Related structures: EMD-23591

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-[(E3-independent) ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121562 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
Histone H3.1 Chains: A, E
Molecule details ›
Chains: A, E
Length: 140 amino acids
Theoretical weight: 15.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68431 (Residues: 1-136; Coverage: 100%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 107 amino acids
Theoretical weight: 11.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62805 (Residues: 1-103; Coverage: 100%)
Gene names: H4-16, H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2B type 1-J Chains: D, H
Molecule details ›
Chains: D, H
Length: 126 amino acids
Theoretical weight: 13.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06899 (Residues: 1-124; Coverage: 98%)
Gene names: H2BC11, H2BFR, HIST1H2BJ
Sequence domains: Core histone H2A/H2B/H3/H4
Breast cancer type 1 susceptibility protein Chain: M
Molecule details ›
Chain: M
Length: 124 amino acids
Theoretical weight: 13.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38398 (Residues: 1-100; Coverage: 5%)
Gene names: BRCA1, RNF53
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Ubiquitin-conjugating enzyme E2 D3 Chain: P
Molecule details ›
Chain: P
Length: 165 amino acids
Theoretical weight: 17.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61077 (Residues: 2-147; Coverage: 99%)
Gene names: UBC5C, UBCH5C, UBE2D3
Sequence domains: Ubiquitin-conjugating enzyme
BRCA1-associated RING domain protein 1 Chain: N
Molecule details ›
Chain: N
Length: 97 amino acids
Theoretical weight: 10.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99728 (Residues: 26-122; Coverage: 13%)
Gene name: BARD1
Sequence domains: zf-RING of BARD1-type protein
Histone H2A type 1-B/E Chains: C, G
Molecule details ›
Chains: C, G
Length: 119 amino acids
Theoretical weight: 13.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04908 (Residues: 13-130; Coverage: 91%)
Gene names: H2AC4, H2AC8, H2AFA, H2AFM, HIST1H2AB, HIST1H2AE
Sequence domains:
DNA (147-MER) Chain: I
Molecule details ›
Chain: I
Length: 147 nucleotides
Theoretical weight: 45.14 KDa
DNA (146-MER) Chain: J
Molecule details ›
Chain: J
Length: 147 nucleotides
Theoretical weight: 45.61 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.28Å
Relevant EMDB volumes: EMD-23591
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The Chromatin Landscape Channels DNA Double-Strand Breaks to Distinct Repair Pathways.
Chen et al. (2022)
11 more

3 mentions without citation

BRCA1/BARD1 is a nucleosome reader and writer.
Witus et al. (2022)
2 more