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Rhodopsin-Gi protein complex

Electron Microscopy
4.38Å resolution
PDB entry 6qno coloured by chain, front view.
PDB entry 6qno coloured by chain, side view.
PDB entry 6qno coloured by chain, top view.
Source organisms:
Assembly composition:
hetero hexamer (preferred)
Entry contents: 6 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 61180.25 Å2
Buried surface area: 14065.77 Å2
Dissociation area: 2,254.51 Å2
Dissociation energy (ΔGdiss): -7.25 kcal/mol
Dissociation entropy (TΔSdiss): 28.71 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-208773

Macromolecules

Guanine nucleotide-binding protein G(i) subunit alpha-1
Chain: A
Length: 376 amino acids
Theoretical weight: 43.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Pfam: G-protein alpha subunit
InterPro:
Guanine nucleotide-binding protein G(i) subunit alpha-1 in PDB entry 6qno, assembly 1, front view.
Guanine nucleotide-binding protein G(i) subunit alpha-1 in PDB entry 6qno, assembly 1, side view.
Guanine nucleotide-binding protein G(i) subunit alpha-1 in PDB entry 6qno, assembly 1, top view.
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Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Pfam: WD domain, G-beta repeat
InterPro:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 6qno, assembly 1, front view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 6qno, assembly 1, side view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 6qno, assembly 1, top view.
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Guanine nucleotide-binding protein G(T) subunit gamma-T1
Chain: G
Length: 74 amino acids
Theoretical weight: 8.56 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02698 (Residues: 1-74; Coverage: 100%)
Gene name: GNGT1
Pfam: GGL domain
InterPro:
Guanine nucleotide-binding protein G(T) subunit gamma-T1 in PDB entry 6qno, assembly 1, front view.
Guanine nucleotide-binding protein G(T) subunit gamma-T1 in PDB entry 6qno, assembly 1, side view.
Guanine nucleotide-binding protein G(T) subunit gamma-T1 in PDB entry 6qno, assembly 1, top view.
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Fab antibody fragment light chain
Chain: L
Length: 239 amino acids
Theoretical weight: 26.31 KDa
Source organism: Mus musculus
Expression system: hybrid
InterPro:
Fab antibody fragment light chain in PDB entry 6qno, assembly 1, front view.
Fab antibody fragment light chain in PDB entry 6qno, assembly 1, side view.
Fab antibody fragment light chain in PDB entry 6qno, assembly 1, top view.
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Fab antibody fragment heavy chain
Chain: H
Length: 249 amino acids
Theoretical weight: 26.56 KDa
Source organism: Mus musculus
Expression system: hybrid
InterPro:
Fab antibody fragment heavy chain in PDB entry 6qno, assembly 1, front view.
Fab antibody fragment heavy chain in PDB entry 6qno, assembly 1, side view.
Fab antibody fragment heavy chain in PDB entry 6qno, assembly 1, top view.
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Rhodopsin
Chain: R
Length: 348 amino acids
Theoretical weight: 39.04 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Pfam:
InterPro:
Rhodopsin in PDB entry 6qno, assembly 1, front view.
Rhodopsin in PDB entry 6qno, assembly 1, side view.
Rhodopsin in PDB entry 6qno, assembly 1, top view.
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