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Apo PheRS from P. aeuriginosa

X-ray diffraction
2.79Å resolution
PDB entry 4p71 coloured by chain, front view.
PDB entry 4p71 coloured by chain, side view.
PDB entry 4p71 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Phenylalanine--tRNA ligase
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Multimeric state: hetero tetramer
Accessible surface area: 82232.47 Å2
Buried surface area: 24715.12 Å2
Dissociation area: 5,507.1 Å2
Dissociation energy (ΔGdiss): 38.36 kcal/mol
Dissociation entropy (TΔSdiss): 17.23 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-191226

Macromolecules

Phenylalanine--tRNA ligase beta subunit
Chains: A, B
Length: 792 amino acids
Theoretical weight: 86.9 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I0A4 (Residues: 1-792; Coverage: 100%)
Gene names: PA2739, pheT
Pfam:
InterPro:
CATH:
Phenylalanine--tRNA ligase beta subunit in PDB entry 4p71, assembly 1, front view.
Phenylalanine--tRNA ligase beta subunit in PDB entry 4p71, assembly 1, side view.
Phenylalanine--tRNA ligase beta subunit in PDB entry 4p71, assembly 1, top view.
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Phenylalanine--tRNA ligase alpha subunit
Chains: C, D
Length: 338 amino acids
Theoretical weight: 38.11 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I0A3 (Residues: 1-338; Coverage: 100%)
Gene names: PA2740, pheS
Pfam:
InterPro:
CATH: Bira Bifunctional Protein; Domain 2
Phenylalanine--tRNA ligase alpha subunit in PDB entry 4p71, assembly 1, front view.
Phenylalanine--tRNA ligase alpha subunit in PDB entry 4p71, assembly 1, side view.
Phenylalanine--tRNA ligase alpha subunit in PDB entry 4p71, assembly 1, top view.
Molecule details ›

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